Ela Elyada

I did not get it. How do we use 'reverse transcription' (mRNA-->cDNA-->PCR) on a DNA template?

Reverse transcriptases can use both RNA and DNA as template. However, not with the same efficiency.

For technical reasons outside the scope of this discussion, I wish to reverse transcribe a ssDNA fragment. The question is, how efficient will this be compared to reverse transcription from an RNA template.

Hello Ela Elyada

I have not tried reverse transcription from a DNA template. Nevertheless, I could refer you to the references below. Maybe these could be helpful.

https://patents.google.com/patent/EP2570487A1/en

Gerard G. F., and D' Alessio J. M., Chapter 6 (73-93) From: Methods in Molecular Biology, Vol.16: Enzymes of Molecular Biology Edited by: M. M. Burell 1993 Humana Press Inc. Totowa, NJ.

Best.

Ela Elyada

Thanks for the answer. Here, it says less efficient.