I am using Taqman to detect a transcript following a knockdown experiment of that gene in cells using esiRNA from Sigma (a mix of 19 siRNA sequences targeting different areas on the transcript of interest). Oddly, I am getting upregulation of this gene instead of downregulation. Anyone encountered this problem using Taqman? I should say that running the same samples using SYBR and standard primers resulted in the expected downregulation, but I am using Taqman to optimize the Real Time detection. Would appreciate any insight!
It appears that some studies show both overexpression and silencing of genes with siRNA.
Morten Lundh et al; 10.1016/j.molmet.2017.07.001
Soo Hyeon Lee et al; 10.1016/j.biomaterials.2010.11.062Farzaneh Modarresi; 10.1038/nbt.2158
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