Ela Elyada

7 Questions 13 Answers 0 Followers

Questions related from Ela Elyada

Has anyone tried reverse transcription from a DNA template?

Has anyone tried reverse transcription from a DNA template? How well does it work compared to an RNA template?

29 March 2022 8,691 4 View

Why my gene is upregulated following an siRNA?

I am using Taqman to detect a transcript following a knockdown experiment of that gene in cells using esiRNA from Sigma (a mix of 19 siRNA sequences targeting different areas on the transcript of...

15 March 2021 3,180 3 View

What is the RNA concentration in cell culture media?

The RNA concentration in serum according to literature is about 5-40 ng/ml. However, there is very little information about the RNA concentration in conditioned medium of cultured cells....

28 June 2019 6,454 7 View

Does MHC-II Ab clone M5/114.15.2 block the receptor?

Hi, I want to sort cells using clone M5/114.15.2 (MHC class II), and then use them in a T cell activation assay. Does anyone know if the bound antibody from sorting will block the MHC-II receptor...

11 April 2018 1,508 6 View

How can I embed a snap frozen tissue in OCT?

I have an already frozen tissue, and I want to embed it in OCT in order to section it for immunostaining. What is the best way to do it without ruining the tissue structure and without subjecting...

18 August 2016 2,289 8 View

Can I inject Brefeldin A i.p.?

Hi, Does anybody know if I can inject Brefeldin A to mice i.p. instead of i.v., and if so, what is the dose?

31 May 2016 179 3 View

What will be the most efficient way to amplify your cDNA of choice for the purpose of over-expressing it later?

Should I use Oligo-dT with a high processivity reverse transcriptase to make the total mRNA and then use specific primers to PCR amplify it? or is there a better way? Thanks

15 January 2015 2,464 3 View