9 Questions 16 Answers 0 Followers
Questions related from Ela Elyada
Has anyone tried reverse transcription from a DNA template? How well does it work compared to an RNA template?
29 March 2022 8,753 4 View
I am using Taqman to detect a transcript following a knockdown experiment of that gene in cells using esiRNA from Sigma (a mix of 19 siRNA sequences targeting different areas on the transcript of...
15 March 2021 3,278 3 View
The RNA concentration in serum according to literature is about 5-40 ng/ml. However, there is very little information about the RNA concentration in conditioned medium of cultured cells....
28 June 2019 6,542 7 View
Will be glad to get some details about this procedure: which Abs to use and how much to use? should I incubate the APCs with the Abs before I introduce the T cells or at the same time? Many thanks...
28 June 2019 1,989 2 View
Hi, I want to sort cells using clone M5/114.15.2 (MHC class II), and then use them in a T cell activation assay. Does anyone know if the bound antibody from sorting will block the MHC-II receptor...
11 April 2018 1,593 6 View
I am looking for Cre-ERT expression in WB in cells, using Cre Ab. Cre itself is 38 kDa but I'm getting a band higher than 50 kDa
13 August 2017 2,526 2 View
I have an already frozen tissue, and I want to embed it in OCT in order to section it for immunostaining. What is the best way to do it without ruining the tissue structure and without subjecting...
18 August 2016 2,340 8 View
Hi, Does anybody know if I can inject Brefeldin A to mice i.p. instead of i.v., and if so, what is the dose?
31 May 2016 254 3 View
Should I use Oligo-dT with a high processivity reverse transcriptase to make the total mRNA and then use specific primers to PCR amplify it? or is there a better way? Thanks
15 January 2015 2,539 3 View
