Hi Ela, if you look on the reagent page https://www.thermofisher.com/antibody/product/MHC-Class-II-I-A-I-E-Antibody-clone-M5-114-15-2-Monoclonal/17-5321-81

under product specific information. It does state that the Ab has been reported to inhibit I-A restricted T cell responses.

Hope this helps...

Dear Ela,

Which receptor do you mean?

The T-cell receptor, CD4 or another receptor?

I would guess you mean the T-cell receptor.

Because the antibody recognize all HLA class II molecules, I would assume, that this antibody is directed against conserved regions of the HLA class II molecules outside the T-cell receptor binding side.

Please have a look to:

https://www.thermofisher.com/antibody/product/MHC-Class-II-I-A-I-E-Antibody-clone-M5-114-15-2-Monoclonal/12-5321-81 and

Article Analysis of T‐cell hybridomas with an unusual MHC class II‐d...

Best regards,

Thomas

Hi Thomas and Shen An,

Thanks for your replies. I read the product webpage, but it wasn't clear and there is only one study done showing this. I wondered if anybody has their own experience with it. Thomas, I didn't mean the TCR or CD4, I meant the MHC-II receptor itself, i.e. will the MHC-II Ab I use for sort block the MHC-II interaction with T cells? Thanks!

Dear Ela,

I am not sure, if I understand your question on the right way. ?:-(

There is a direct interaction between the peptide binding side of the MHC class II molecules on your (human ?) cells and the T-cell receptor on T-cells. There is direct interaction between CD4 and an other part of the HLA-class II, too.

I would expect, that you can not block these both inreactions with this antibody.

Best regards,

Thomas

Your T cell activation assay will operate over the direct interaction between the HLA-class I petide bonding side on your cells and the T-cell receptor on the T-cells!

I would assume, that this antibody will not disturb this interaction!

Thomas

Yes, but blocking either of those interactions will hamper my T cell response, so I can get a negative result when actually this is a technical issue. That is my worry.

Thanks for your input though.