Has anyone made transformation by using Trans-Blot® SD Semi-Dry Transfer Cell equipment from BioRad using 2D acrylamide gels 1.5mm?

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My question concerns MTT cell viability assay?

Despite not having cells in the media, I am getting purple color. I have tried many troubleshooting methods, varying media types, and even different MTTs from different companies to figure out the...

21 July 2024 9,914 1 View

Guidance needed for preparing the hydrogel samples for the XRD instrument?

I'm working with a hydrogel sample and I'd like to perform XRD analysis. Can anyone offer guidance on preparing the hydrogel for the XRD instrument? Specifically, I'm unsure about the best method...

20 July 2024 3,611 4 View

How can I extract the mathematical equation from existing Neural Network Model?

There exists a neural network model designed to predict a specific output, detailed in a published article. The model comprises 14 inputs, each normalized with minimum and maximum parameters...

14 July 2024 2,714 3 View

Why does my IVT gRNA product gives HMW smear in gel?

We are using MEGAshortscript™ T7 Transcription Kit for transcribing gRNAs from linearized plasmid templates. (MSP3485 digested with HindIII) (https://www.addgene.org/140082/) We let the reaction...

01 July 2024 6,342 0 View

How can Abaqus CAE be utilized to assess the variation in wall thickness throughout a plastic bottle following a blow molding simulation?

Hello ResearchGate Community, How can Abaqus CAE be utilized to assess the variation in wall thickness throughout a plastic bottle following a blow molding simulation?

26 June 2024 9,281 4 View

JCR 2024 Impact Factor Download File-Factors caused decline in IF are?

Why the IF of almost all journals declined so much? For the file download go to https://www.researchgate.net/post/JCR_Impact_Factor_2024-Factors_causes_decline_in_IF

21 June 2024 3,358 0 View

When use a HOMER Pro to design hybrid system PV/electrolyzer/hydrogen tank. How can I use all electrical load to power the electrolyzer?

Simulating a Hybrid System with PV, Grid, Electrolyzer (No Load) in HOMER. here is a question about how to use HOMER Pro to design a solar PV system for water electrolysis using electrolyzer for...

05 June 2024 5,609 0 View

Expert questionnaire, Is it possible for people with a specialty in music psychology to evaluate this questionnaire? Through the link below?

It is possible for people specialized in music psychology to evaluate this questionnaire Through the link below Thank you https://forms.gle/qzoD7jN2UDUhMjwQ7

02 June 2024 9,546 0 View

How can we extract DNA from yeast isolates without using any lysis buffer or lysis enzymes? Is there any manual protocol?

31 May 2024 548 4 View

Please could colect all my publications under my name ?

please could collect all my publications under my name ?

30 May 2024 2,891 1 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Why I can't see any band in SDS-PAGE?

Currently, when I run SDS-PAGE, I don't see any bands at all, even though I used the same material just a day ago and it worked fine.... In our lab, we dilute the 10X running buffer to 1X and...

06 August 2024 5,373 2 View

Why is my ASO degraded quickly on agarose gel?

I have been working on Red blood cell-derived extracellular vesicles as Antisense Oligonucleotide (ASO) carriers. We normally run agarose gel to quantify the loading efficiency. I used naked ASO...

06 August 2024 3,130 2 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

How to preform densitometry on SDS-page bands?

I ran a SDS-page of a bacterial lysate and I want to quantify protein concentration in a specific band. I was thinking of using a standards ladder or make some standards are different...

05 August 2024 9,805 3 View

How to use Density Functional Theory to calculate carrier mobilities of solid system?

Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...

04 August 2024 8,894 1 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

How can I get my Granzyme B flow cytometry stain to be consistent?

I have used PE and PE-Dazzle 594 fluorochromes and have managed to get NK cells to properly show GranzymeB expression after 4 hr PMA/ionomycin stimulaton, but for some reason my CD8 cells in the...

01 August 2024 7,677 2 View

I'm optimizing a tetra-ARMS PCR protocol with amplicon sizes: 165bp, 123bp and 93bp. Why, in the gel, only 165bp is visible while I'm expecting all 3?

It's an end-point PCR protocol. I'm using 1.5% agarose gel with SyBR Safe dye and TBE as a running buffer, visualization on BioRad XR+ system. I was primarily thinking of primer efficiency,...

01 August 2024 4,673 4 View