Purified protein requires Calcium for activation, but the elution buffer I used seems to interact with Calcium, help?

09 February 2021 6,946 4 View

Can someone explain blocking vs. nonblocking electrodes in terms of electrochemical impedance spectroscopy?

I have fabricated thin films and want to measure the impedance parallel to the material. I suspect the films are conducting protons and electrons based on some Hall effect measurements I have...

27 January 2021 1,712 5 View

Isoabsorbance and 3D Plots are missing under the "Spectra" toolbar of Agilent Chemstation 1200 series software. How can I retrieve it please?

The Problem started after replacing the HPLC Pump from Quaternary to Isocratic pump due to pump failure. The configuration was set as the previous set up as Modular 3D LC System and the license...

11 October 2020 8,018 3 View

I am using a rat TNFa ELISA to measure TNFa in culturing media. How soon following my alcohol treatment should I collect and measure TNFa?

I am using an organotypic hippocampal slice culture where we are exposing our slices to alcohol with subsequent alcohol withdrawal. I want to make sure I am capturing the right time to assess...

16 September 2020 7,020 2 View

When is simulation necessary in copula modeling?

When modeling dependencies using copulas, when is simulation necessary? Kindly need expert advice on this.

08 June 2020 7,166 3 View

Has anyone using organotypic hippocampal slice culture models observed holes in their tissue specimens between 6-12 days in vitro?

Our lab has noticed that after several tissue preparations for organotypic hippocampal slice cultures, some of our tissue is developing what appears to be large holes between the CA1 and dentate...

03 March 2020 3,981 3 View

How do I graph RT-PCR amplification values?

I have conducted an experiment to investigate the up-regulation and down-regulation of immune related genes. I have calculated the PCR efficiency and used the pfaffl method to get amplification...

22 October 2019 1,231 1 View

Beta-actin antibody for western blot loading control is too strong?

While the data sheet for the beta-actin antibody I use (see attached) suggests a dilution range of 1:100-1:1000, I've found that following this recommendation leaves me with bands so thick and...

19 July 2019 2,770 4 View

Is Learning Analytics under Data Mining?

In what ways EDM and LA differ and does LA under Data Mining and does it use the same techniques from Data Mining?

30 April 2019 8,370 1 View

No title

13 December 2017 8,042 3 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

What element is driving the different slop in the low frequency tail?

My study consists simple corrosion inhibitors in salty brine. Right now I just fit my circuit with randles with CPE. Just wondering how the slop can given more info of the experiment. Or any...

01 March 2021 6,474 3 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Why does intensity drops as concentration increases?

Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?

28 February 2021 3,653 4 View

How to calculate Ligand-active site distance in a ligand-protein complex from MD simulation?

I have run a MD simulation of a ligand-protein complex in GROMACS using CHARMM forcefield. Reviewer has asked me to calculate Ligand-activesite distance and binding free energy of ligand with...

27 February 2021 4,703 1 View