We're following the recipe from the Invitrogen NuPAGE Tech Manual [50 mM MOPS, 50 mM Tris Base, 0.1% SDS, 1 mM EDTA pH 7] but the solution is still cloudy, even after applying heat. We haven't had this problem before. Thanks!
What is the pH? Some buffers don't go into solution until you get them close to the pH of interest....
pH 7.0. We tweaked the pH a little and that helped. Thanks!
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