10 October 2015 5 851 Report

Our -80C freezer broke and we're not sure if we can safely move them to our -150C freezer which can be set as low as -125C.

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Has anyone had trouble getting MOPS to go into solution [50 mM for 20X SDS-PAGE Running Buffer]?

We're following the recipe from the Invitrogen NuPAGE Tech Manual [50 mM MOPS, 50 mM Tris Base, 0.1% SDS, 1 mM EDTA pH 7] but the solution is still cloudy, even after applying heat.  We haven't...

01 February 2016 2,129 2 View

Reasons for anomalous migration of high MW DNA on agarose gels?

We have been having consistently anomalous migration of high / higher MW DNA on our agarose gels.  When running a linearized 5.3 kb plasmid, it's running above the 10 kb MW standard.  However,...

01 February 2016 6,208 5 View

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