What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

Are you interested in the conference 'Sport and crisis: bodies, practices, representations'?

Dear sport sociologists, you can find below the Call for Papers of the Conference - Sport and crisis: bodies, practices, representations. The conference will be held in a hybrid form at the...

30 November 2020 460 7 View

I can't make my RAPD-PCR work anymore. Can anyone help me?

Guys, I REALLY NEED HELP At the end of the last year I optimized every parameter of my RAPD-PCR, and as a result I did obtain great products multiple times. Then, I went home for my vacation (I...

18 February 2020 9,236 3 View

What else other than protein absorbs at 280nm in my sample when meassuring protein concentration at nanodrop?

Dear all, in our lab we are working on Outer Membrane Vesicles (OMVs). When we purify these vesicles we simply quantify the total protein content using nanodrop. Then we check the quality of the...

26 February 2018 4,800 10 View

Does anyone have or is aware of literature or data regarding the surplus power generation in solar and wind farms?

Hello, I am looking at possible ways to store and use the surplus power generation from renewable energy plants, solar and wind farms above all. I would be willing to collect some data first, so...

02 November 2017 6,830 3 View

How can I remove a low-copy plasmid from E. coli?

Dear all, in my lab we are working with E. coli. A couple of weeks ago we tried to remove a low-copy plasmid (pACYC) from one of our strains by simply growing it in the absence of the selective...

17 October 2017 9,398 2 View

Live/Dead assay on fixed tissue/cells?

Hi all, is it possible to perform a Live /Dead assay on fixed tissue using Pi and FDA or any other dye? Thank you in advance

14 March 2017 5,077 1 View

How can I find out if variable A moderates the effect between variable B and C using SPSS?

I already proved a negative relationship between variable B and C, however my hypothesis in my research paper is that variable A moderates the negative effect of variable B on C. I read a number...

06 December 2016 2,698 4 View

How do endocannabinoids modify the immunohistochemical expression of CB1 (receptor) and FAAH (enzyme)?

The increase of the ligand increases the receptor and the enzyme or only one or the other.

14 April 2013 8,452 3 View

Anyone plz suggest relevant topic for research in image processing+Deep learning.?

I have selected brain tumor images ...but now found that already lots of research done n this topic.

03 March 2021 5,774 3 View

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02 March 2021 3,060 3 View

How can we calculate the sample size for rodents for a particular period of time?

How can we calculate the sample size for rodents for a study for one year? Are there any specific protocols to be followed for designing rodent sampling? How long should be the sampling interval...

01 March 2021 1,056 3 View

Would fecal samples stored at -20C for over a year impact metagenomic analysis?

Dear All, I have access to some rodent fecal samples that have been stored at -20C instead of -80C. Anyone have experience with the impact of stowage condition on comparative metagenomic analysis...

01 March 2021 2,512 1 View

What kind of food stuff increase the potentiality of human brain?

There are different kinds of food stuffs which we intake daily according to our requirements. Carbohydrate, protein, fat, mineral, vitamin, water are mainly working for the growth and development...

01 March 2021 831 2 View

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28 February 2021 9,936 2 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Can Ethovision store the generated data and video recordings directly to NAS system?

The size of data generated by Ethovision is huge. Having NAS system for data storage seems to be a good option, I am wondering whether Ethovision can directly store the data to the NAS system...

27 February 2021 6,350 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View