What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

Are you interested in the conference 'Sport and crisis: bodies, practices, representations'?

Dear sport sociologists, you can find below the Call for Papers of the Conference - Sport and crisis: bodies, practices, representations. The conference will be held in a hybrid form at the...

30 November 2020 460 7 View

I can't make my RAPD-PCR work anymore. Can anyone help me?

Guys, I REALLY NEED HELP At the end of the last year I optimized every parameter of my RAPD-PCR, and as a result I did obtain great products multiple times. Then, I went home for my vacation (I...

18 February 2020 9,236 3 View

What else other than protein absorbs at 280nm in my sample when meassuring protein concentration at nanodrop?

Dear all, in our lab we are working on Outer Membrane Vesicles (OMVs). When we purify these vesicles we simply quantify the total protein content using nanodrop. Then we check the quality of the...

26 February 2018 4,800 10 View

Does anyone have or is aware of literature or data regarding the surplus power generation in solar and wind farms?

Hello, I am looking at possible ways to store and use the surplus power generation from renewable energy plants, solar and wind farms above all. I would be willing to collect some data first, so...

02 November 2017 6,830 3 View

How can I remove a low-copy plasmid from E. coli?

Dear all, in my lab we are working with E. coli. A couple of weeks ago we tried to remove a low-copy plasmid (pACYC) from one of our strains by simply growing it in the absence of the selective...

17 October 2017 9,398 2 View

Live/Dead assay on fixed tissue/cells?

Hi all, is it possible to perform a Live /Dead assay on fixed tissue using Pi and FDA or any other dye? Thank you in advance

14 March 2017 5,077 1 View

How can I find out if variable A moderates the effect between variable B and C using SPSS?

I already proved a negative relationship between variable B and C, however my hypothesis in my research paper is that variable A moderates the negative effect of variable B on C. I read a number...

06 December 2016 2,698 4 View

How do endocannabinoids modify the immunohistochemical expression of CB1 (receptor) and FAAH (enzyme)?

The increase of the ligand increases the receptor and the enzyme or only one or the other.

14 April 2013 8,452 3 View

How can you bring two structures together to create an adhesive bond in LAMMPS?

I have created an Ice 1h crystalline structure and an Aluminium substrate structure and equilibrated both at 250K. Now I need to bring them together in a way that an adhesive bond is created....

01 March 2021 3,325 2 View

Necessary tube lenght to cool Natural gas in a dry-ice cooling bath?

I have a tank which contains natural gas at 250 Bar and 293 Kelvin. I want to cool the gas in a dry-ice cooling bath to about 195 Kelvin. The gas will flow in a copper-tube through the bath with a...

27 February 2021 8,128 1 View

Where i will get the Desmodium and Napier grass seeds?

26 February 2021 975 4 View

How to raise temperature in small increments during equilibration in LAMMPS?

All, I am trying to equilibrate an Ice 1h crystalline structure to 250K in LAMMPS. When I tried to do this at 250K directly, the structure lost it's crystalline shape and became disordered post...

14 February 2021 5,747 2 View

Stats to compare recharge of groundwater and determine if precipitation has more of an effect on groundwater levels before or after resto??

Hello all, I am looking for ways to compare recharge of groundwater and determine if precipitation has more of an effect on groundwater levels before or after restoration? My data set contains a...

08 February 2021 7,887 5 View

Prompt research in the direction of "deprivation and adolescents" please?

01 February 2021 6,401 3 View

Habitat Suitability through GIS without presence-absence data?

What kind of models/techniques can be used with GIS spatial analysis in order to determine habitat suitability for a species that is not (widely) present in a certain region? For my bachelor...

31 January 2021 2,777 2 View

Should the Ice 1h crystalline structure be lost during equilibration process in LAMMPS?

I have created an Ice 1h structure and am equilibrating it 100K in NVT ensemble in LAMMPS for a timestep of 0.25fs in 3000 steps. The potential used is TIP4P/Ice. Although the simulation runs fine...

26 January 2021 9,603 11 View

Strong and extreme winds in the Arctic?

Hello everybody! Looking for a good source of information on strong and extreme winds in the Arctic (monthly values, annual extremes etc.). The form of maps (with isolines) will be most...

25 January 2021 8,984 4 View

How to remove a sapphire disk from LR white resin embedding ?

Hello, We tried to do CLEM experiment on sapphire disk including cryo fixation (Leica ICE) and then cryo substitution using LR white resin. Once the resin is polymerized, we can't remove the...

13 January 2021 5,223 3 View