I have never seen like this. Were they growing first 9 days well?

Dear Sumanta,

It was documented that temperature and calcium ions affect aggregation of mesenchymal stem cells in phosphate buffered saline.

For more details please see the attached publication.

Hoping this will be helpful,

Rafik

Dear Sumanta,

It was documented that temperature and calcium ions affect aggregation of mesenchymal stem cells in phosphate buffered saline.

For more details please see the attached publication.

Hoping this will be helpful,

Rafik

Yes. They were growing and exhibiting the morphology as they should. But, all of a sudden they started to form aggregates. 

Thank you, rafik.

yes, it happened with me many a times when the culture grows old.

Thanks, parinita. So how exactly did you get rid of the problem ? You had to passage the cells or change the media ?