How to analyse GC MS spetra?

Kindly help to interpret GC-MS of plant extract? how to relate a particular compound from the spectra. What is important point to consider? Thank you all

09 October 2018 7,321 5 View

What gene will be suitable for molecular Plant identification?

Dear researcher, I would like to identify and confirm plants by molecular method. So, what gene will be best suited for this purpose?

08 September 2018 5,134 0 View

Can anyone suggest AntiCancer research approach?

Dear Experts, I would to carry out a research on Plant source medicinal or ethno-pharmaceutical in vitro and in vivo. I would to focus on Biochemical-Physiological profiling and molecular...

06 July 2018 7,448 0 View

Can cancer cells from animal origin develop in human?

Can cancer cells from animal origin growth, develop and proliferate in human? What if not, what exactly is the reason? is it immune system incompatible alone for not proliferation? Thanks

07 August 2016 6,356 1 View

Can ulcer be induced in short time by chemical?

Can ulcer be induced and treated well in hours? As far my understanding is concern, it is chronic symptom. Does the healing of ulcer involves division of cell or tissue formation? Thanks

07 August 2016 1,726 1 View

Is it sufficient anova way statistical analysis?

Dear researchers, I need to perform biochemical experiment data to analysis statistical significance. I have more two groups for all experiments. But I am not sure what statistical parameter will...

03 April 2016 1,818 3 View

Can anyone share me some information on western blot?

Respected Researchers, I would like to know what is the principle behind the Western blotting? How will be the expression of interest protein if treated cancer cell with drugs? I read many...

03 April 2016 3,759 2 View

Kindly help me with western blotting?

Researchers, can anyone suggest me western blotting technique? I need to perform western blotting but i have no practical experienced yet. How to develop the band or expression of protein on...

02 March 2016 3,458 6 View

What stastistical parameters will be appropriate for biochemical assays?

02 March 2016 1,113 6 View

How acurate is Acridine orange/ ethidium bromide detection of apotosis and micronuclei?

I am working on plant based compound activity on human cancer cell lines. I would like to observe any damage to DNA but i am confused what experiment will be appropriate. Can anyone share me...

01 February 2016 6,592 5 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

No title

02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View