I am currently working on A431 cell lines. It seems that the doubling time is 18-20 hrs but online information showed higher than what i am observed.
Can anyone share me information regarding A431 cell lines?
Thank you,
Kindly help to interpret GC-MS of plant extract? how to relate a particular compound from the spectra. What is important point to consider? Thank you all
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Dear researcher, I would like to identify and confirm plants by molecular method. So, what gene will be best suited for this purpose?
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Can cancer cells from animal origin growth, develop and proliferate in human? What if not, what exactly is the reason? is it immune system incompatible alone for not proliferation? Thanks
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Can ulcer be induced and treated well in hours? As far my understanding is concern, it is chronic symptom. Does the healing of ulcer involves division of cell or tissue formation? Thanks
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Dear researchers, I need to perform biochemical experiment data to analysis statistical significance. I have more two groups for all experiments. But I am not sure what statistical parameter will...
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Researchers, can anyone suggest me western blotting technique? I need to perform western blotting but i have no practical experienced yet. How to develop the band or expression of protein on...
02 March 2016 3,647 6 View
Dear Researchers, I need to analyse biochemical experiments and i am confused which parameters will i use. 1. I performed in vivo and in vitro antioxidant enzyme activity of different treatment...
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I am working on plant based compound activity on human cancer cell lines. I would like to observe any damage to DNA but i am confused what experiment will be appropriate. Can anyone share me...
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I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
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Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
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I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
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I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
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I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
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Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
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I am currently looking for a questionnaire that relates with my study which is The Effectiveness of Organizational Culture towards employee retention rates
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I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...
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