Hallo! Can you please give me some insights of 'why breast cancer cells are cultured in 12 days with exogenous ECM?'

More Melvin Ayes Bagot's questions See All

Are you interested in doing a Postdoc at University of Pretoria?

Please see postdoc funding opportunities available at University of...

19 November 2023 8,672 1 View

Are constructed wetlands better alternatives for wastewater treatment?

How can constructed wetlands be used as wastewater treatment

27 September 2023 5,302 6 View

Why the disturbed mangrove forest in Barangay Molugan six different kinds of bats had while the other sampling stations only had four?

Can someone help me interpret the data that I have recently collected? I am studying bat diversity in three selected mangrove forests in Misamis Oriental and Northern Mindanao. Here are the data...

17 March 2023 6,537 2 View

Why are perivascular space important?

Recently, I was introduced to perivascular spaces (PVS) while studying lipopolysaccharide treated models with transmission electron microscopy (TEM). Erickson MA, Shulyatnikova T, Banks WA,...

16 February 2023 2,519 7 View

Storing protein lysates in sample buffer or not?

We have our rat brain lysates stored at -80C and are about to start Westerns. We will need to thaw them for BCA quantification, but then want to have several aliquots of this available in the...

31 January 2023 3,048 2 View

Logistic regression and Risk estimate analysis?

Dear Scholars, I am currently working on HPV and cervical cancer self-reporting data. I want to evaluate the risk values of the population using the risk factors of the diseases mentioned...

12 October 2022 4,330 1 View

Do you have any thoughts regarding the importance of folate-mediated one--carbon metabolism in COVID 19?

Soon I will be writing a paper on IMPAIRED Folate-mediated One-Carbon Metabolism (FOCM) in COVID-19, and I will be needing input from a vast array of scientists and researchers who are interested...

25 September 2021 4,517 3 View

Data Analysis K-S and S-W tests?

Hello, I have conducted analysis where the K-W and S-W have come out as p

25 February 2021 10,015 3 View

Does anyone have new information regarding the role of a low High Density Lipoprotein in the association of abdominal aortic aneurysm?

I have a 76 y/o male patient with history of low HDL-C who had an myocardial infarction in 1997. He has done well following a program of dietary changes and exercise but has not quit smoking. He...

21 January 2021 6,082 4 View

SARS-CoV-2 Viremia: How do pulmonary infections advance to extrapulmonary manifestations in COVID-19?

The process of viremia involving the transfer of the whole virus from pulmonary alveolar epithelial pneumocytes to the alveolar capillaries and hence to the systemic circulation is not well...

07 October 2020 923 6 View

Which Scopus Journal provides the most affordable fees?

"PUBLISHING IN A SCOPUS JOURNAL" Researchers are now at a cross road. The critical need to publish in a Scopus or ISI, etc journal is ever vital. Journal Publication fees must be submitted....

10 August 2024 8,621 1 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is this a facetotecta nauplius?

This larva was captured using a plankton net in the Persian Gulf during the summer. I believe it may be a Facetotecta nauplius.

08 August 2024 3,746 4 View

GC-MS retention index prediticon?

Hello experts, Does anyone know any free software about retention index prediction ?

08 August 2024 7,403 2 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Could you please suggest methods to compare free protein and immobolized protein binding properties?

I have an antibody binding generic protein and I need to compare its activity in a free and immobolized form. I understand that there are a number of methods to determine Kd value of a free...

05 August 2024 5,311 0 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View