Does any one know about HES staining??

Actully I want to use Hemtoxylene eosin and safran (HES) staining for my bone tissue sections , can anybody guide me regarding its protocol??

03 April 2019 10,024 1 View

Can any one guide me in explainng this picture??

HI everyone, I just isolated my RNA from directly from blood by TRIZOL method, I got purity of 1.97 (260/280) which is pretty in acceptable region, but when I run my RNA on gel it shows 3 bands....

31 December 2018 7,760 8 View

What should be the dissociation curve in real time PCR if my primer has similarity with its own transcript variance?

If my primer has the specificty with transcript variance of its own, then what should be the the possiblity dissociation curve of my real time pcr and how its effects my results?

01 February 2018 7,272 4 View

Protocol for 20s proteosomal activity assay??

what is the protocol or method to check the ubiquitin proteosomal in activity in brain samples as i also have flourogenic AMC as substrate for proteosome.

11 December 2017 9,596 0 View

Can anyone tell me how to calculate dilution factor ?

08 September 2017 3,174 5 View

Primer designing

can anyone tell me what would be the change in the bands on gel if i use primers from exon-exon junctions and non exon-exon junction??

07 August 2017 7,628 5 View

Bouine's fixative??

Can any one tell me how to prepare bouine's fixative in lab form powder picric acid and 37% formaldehyde???

02 March 2017 3,682 1 View

Does anyone have a detailed protocol for isolation of SNc from mice brain?

10 November 2016 6,844 3 View

What is the effect on PCR results if two PRIMERS have different GC %??

Read 4 answers by scientists with 4 recommendations from their colleagues to the question asked by Zaid abdul razzak on Oct 24, 2016

09 October 2016 9,394 4 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

What analysis to choose with a large neste dataset and clearly skewed or kurtotic distributions?

Hello! I have a dataset of n=3000 nested within 8 countries with approximately 200 or 400 responses in each country. I originally planned to perform multilevel modelling with 4 dependent variables...

02 March 2021 6,865 1 View

How can we calculate the sample size for rodents for a particular period of time?

How can we calculate the sample size for rodents for a study for one year? Are there any specific protocols to be followed for designing rodent sampling? How long should be the sampling interval...

01 March 2021 1,056 3 View

Would fecal samples stored at -20C for over a year impact metagenomic analysis?

Dear All, I have access to some rodent fecal samples that have been stored at -20C instead of -80C. Anyone have experience with the impact of stowage condition on comparative metagenomic analysis...

01 March 2021 2,512 1 View

How to plot the probability paper of Gumbel distribution using R programing?

In R-studio, there are many commands of Gumbel package. Arguments are also different. I`m asking about the alpha parameter of the Copula which must be greater than 1. If this is the one used to...

25 February 2021 5,229 3 View

What are the pros and cons for mice versus rats?

Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...

24 February 2021 1,825 3 View

Are there methods for automatically mapping floodplains, fans and terraces?

I want to map these river geoforms with as little subjectivity as possible. I have a high resolution DEM generated by drone data.

22 February 2021 7,800 3 View

What should be the duration of exposure to simulated space environments with Rodents?

Hi, I am currently working on a pilot study to get some data related to the impact of long duration spaceflight to the microbiome and the immune system. I plan to use rats and simulate...

22 February 2021 592 1 View

Do I need to introduce HFD to ob/ob mice to obtain a T2DM model?

I am planning to work on T2 diabetic animals and check the efficacy of probiotics. My initial plan is to fed B6 animals with HFD for a while. But now, I have the chance to work on ob/ob mice. My...

18 February 2021 6,896 3 View

What are the options for global scale hydrology DEM?

Hello, I am working on a global scale hydrology GIS project delineating global watersheds and doing some data analysis. I have in the past used hydro1K from the USGS that cover the entire world....

18 February 2021 5,109 5 View