Does any one know about HES staining??

Actully I want to use Hemtoxylene eosin and safran (HES) staining for my bone tissue sections , can anybody guide me regarding its protocol??

03 April 2019 10,024 1 View

Can any one guide me in explainng this picture??

HI everyone, I just isolated my RNA from directly from blood by TRIZOL method, I got purity of 1.97 (260/280) which is pretty in acceptable region, but when I run my RNA on gel it shows 3 bands....

31 December 2018 7,760 8 View

What should be the dissociation curve in real time PCR if my primer has similarity with its own transcript variance?

If my primer has the specificty with transcript variance of its own, then what should be the the possiblity dissociation curve of my real time pcr and how its effects my results?

01 February 2018 7,272 4 View

Protocol for 20s proteosomal activity assay??

what is the protocol or method to check the ubiquitin proteosomal in activity in brain samples as i also have flourogenic AMC as substrate for proteosome.

11 December 2017 9,596 0 View

Can anyone tell me how to calculate dilution factor ?

08 September 2017 3,174 5 View

Primer designing

can anyone tell me what would be the change in the bands on gel if i use primers from exon-exon junctions and non exon-exon junction??

07 August 2017 7,628 5 View

Grip strength test in rodents??

I have an older version of grip strength meter(GSM) UGo basile cat # 47105-001 can any one tell me how i can use it?? Actually its user mannual have lost and i cann't find user mannual of this on...

02 March 2017 7,321 0 View

Does anyone have a detailed protocol for isolation of SNc from mice brain?

10 November 2016 6,844 3 View

What is the effect on PCR results if two PRIMERS have different GC %??

Read 4 answers by scientists with 4 recommendations from their colleagues to the question asked by Zaid abdul razzak on Oct 24, 2016

09 October 2016 9,394 4 View

What is the status of bread making and more bakery foods with the use of insect flour/powder?

Insects have been consumed by human diet since prehistoric times. At present, the idea of looking at insects as food has been getting traction worldwide, also in response to increasing food...

03 March 2021 6,223 5 View

Chlorinated organic formation in odor control scrubbers using hypochlorite?

I'm involved in a study of odor control technologies for municipal wastewater treatment plant. One of the control options involves a chemical 2-stage (acid/alkaline) packed bed scrubber. The...

03 March 2021 3,661 2 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

Energy minimization in LAMMPS using NVT ?

How do I do energy minimization in lammps with NVT ensemble ? I am using the following command and it does not seem to work. It stops at extremely high energy and gives me the error zero search...

01 March 2021 2,294 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How can I edit suggested publications?

Hi there! I regularly receive suggestion about papers and authors that are totally out of my area. Does anybody know how I can fix this? Thank you!

01 March 2021 9,574 4 View

Samples to study circular dichroism (chirality)?

I want to build a low-cost polarimeter to study circular dichroism (chirality). What type of samples can I use? I am looking for samples that can be found outside the laboratory.

01 March 2021 9,101 3 View