Can any one guide me in explainng this picture??

HI everyone, I just isolated my RNA from directly from blood by TRIZOL method, I got purity of 1.97 (260/280) which is pretty in acceptable region, but when I run my RNA on gel it shows 3 bands....

31 December 2018 7,760 8 View

What should be the dissociation curve in real time PCR if my primer has similarity with its own transcript variance?

If my primer has the specificty with transcript variance of its own, then what should be the the possiblity dissociation curve of my real time pcr and how its effects my results?

01 February 2018 7,272 4 View

Protocol for 20s proteosomal activity assay??

what is the protocol or method to check the ubiquitin proteosomal in activity in brain samples as i also have flourogenic AMC as substrate for proteosome.

11 December 2017 9,596 0 View

Can anyone tell me how to calculate dilution factor ?

08 September 2017 3,174 5 View

Primer designing

can anyone tell me what would be the change in the bands on gel if i use primers from exon-exon junctions and non exon-exon junction??

07 August 2017 7,628 5 View

Grip strength test in rodents??

I have an older version of grip strength meter(GSM) UGo basile cat # 47105-001 can any one tell me how i can use it?? Actually its user mannual have lost and i cann't find user mannual of this on...

02 March 2017 7,321 0 View

Bouine's fixative??

Can any one tell me how to prepare bouine's fixative in lab form powder picric acid and 37% formaldehyde???

02 March 2017 3,682 1 View

Does anyone have a detailed protocol for isolation of SNc from mice brain?

10 November 2016 6,844 3 View

What is the effect on PCR results if two PRIMERS have different GC %??

Read 4 answers by scientists with 4 recommendations from their colleagues to the question asked by Zaid abdul razzak on Oct 24, 2016

09 October 2016 9,394 4 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

Does anyone know how to plot my station to a section plot on Ocean Data View (ODV)?

I made a vertical section plot with WOA .nc file on ODV, and now I want to plot my stations (csv. file) in it. Does anyone know how to import my points to the section? They are two different...

01 March 2021 3,610 1 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

How to assign different material properties to each element in Abaqus ?

Hello, I am writing a python script to generate my model and write the input file. The problem is I have 1000 elements in my mesh and I want to assign different material properties to each...

01 March 2021 9,511 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View