Is it necessary to calculate the dilution factor if we dissolve any tissue mass (mg) in liquid volume (ul) with no further dilution ???
Please see following publication on Determination of DNA concentration by Spectrophotometric Estimation. I hope it will serve your purpose.
http://homepages.bw.edu/~mbumbuli/molbio/labs/dna/
Please see following publication on Determination of DNA concentration by Spectrophotometric Estimation. I hope it will serve your purpose.
http://homepages.bw.edu/~mbumbuli/molbio/labs/dna/
According to Regina Edwards, a dilution solution contains solute (or stock solution) and a solvent (called diluent). These two components proportionally combine to create a dilution. You can identify a dilution solution by the amount of solute in the total volume, expressed as a proportion. For example, a chemical may be prepared in a 1:10 dilution of alcohol, indicating that a 10 mL bottle contains one milliliter of chemical and nine milliliters of alcohol. You can calculate the necessary volume of each component to prepare a dilution solution.
Write down the desired final volume of the solution--for example, 30 mL.
Write down the desired dilution in the form of a proportion--for example, 1:20 dilution, also known as the dilution factor.
Convert the dilution factor to a fraction with the first number as the numerator and the second number as the denominator. For example, a 1:20 dilution converts to a 1/20 dilution factor.
Multiply the final desired volume by the dilution factor to determine the needed volume of the stock solution. In our example, 30 mL x 1/20 = 1.5 mL of stock solution.
Subtract this figure from the final desired volume to calculate the volume of diluent required--for example, 30 mL - 1.5 mL = 28.5 mL.
Measure the amount of stock solution required -- in our example, 1.5 mL -- and dispense this into a large measuring cup.
Measure the amount of diluent required -- in our example, 28.5 mL -- and dispense this into the large measuring cup.
Mix the solution with the glass stirring rod. You now have your 1:20 dilution solution.
Dear Jorge
Thanks for your answer , I am using 10 g of tissue in 0.3 ml of liquid with no further dilution so is it necessory to calculate the dilution factor or not ??
Thanks in advance
Regards
zaid
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