what is the protocol or method to check the ubiquitin proteosomal in activity in brain samples as i also have flourogenic AMC as substrate for proteosome.
Actully I want to use Hemtoxylene eosin and safran (HES) staining for my bone tissue sections , can anybody guide me regarding its protocol??
03 April 2019 10,174 1 View
HI everyone, I just isolated my RNA from directly from blood by TRIZOL method, I got purity of 1.97 (260/280) which is pretty in acceptable region, but when I run my RNA on gel it shows 3 bands....
31 December 2018 7,896 8 View
If my primer has the specificty with transcript variance of its own, then what should be the the possiblity dissociation curve of my real time pcr and how its effects my results?
01 February 2018 7,427 4 View
Is it necessary to calculate the dilution factor if we dissolve any tissue mass (mg) in liquid volume (ul) with no further dilution ???
08 September 2017 3,326 5 View
can anyone tell me what would be the change in the bands on gel if i use primers from exon-exon junctions and non exon-exon junction??
07 August 2017 7,775 5 View
I have an older version of grip strength meter(GSM) UGo basile cat # 47105-001 can any one tell me how i can use it?? Actually its user mannual have lost and i cann't find user mannual of this on...
02 March 2017 7,623 0 View
Can any one tell me how to prepare bouine's fixative in lab form powder picric acid and 37% formaldehyde???
02 March 2017 3,823 1 View
does anyone have a detailed protocol for isolation of SNc from mice brain? and which dye is used for stain Dopaminergic neuron cells?
10 November 2016 6,979 3 View
Read 4 answers by scientists with 4 recommendations from their colleagues to the question asked by Zaid abdul razzak on Oct 24, 2016
09 October 2016 9,548 4 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...
06 August 2024 5,801 4 View
Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...
05 August 2024 6,247 3 View
Hi everyone, I am working on brain slices for visualizing a protein in the soma and dendrites, using a fluorescence tag. However, I need a tool (not paid) for reconstruction of the whole neuron,...
04 August 2024 4,725 2 View
I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....
04 August 2024 762 1 View
Do You Know Untreated TMJ Affects Your Brain And Entire Body?
04 August 2024 6,282 1 View
What is the best way to freeze the rat brain - liquid nitrogen, Dry ice , isopentane - after isolation for later protein investigation by Elisa?
01 August 2024 1,722 1 View
I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...
31 July 2024 1,123 4 View
Information in The Three Dimensions of Time.Information in The Three Dimensions of Time Information Transfer based on Brains Entanglement.Information Transfer based on Brains Entanglement Time...
28 July 2024 7,522 0 View
I have found an EEG where only alpha waves are present. Beta waves are not found in active patients. What interpretations ?
26 July 2024 4,741 1 View