FDC and FRC cell populations in mouse spleen?

I was trying to isolate fibroblastic reticular cells (FRC) and follicular dendritic cells from mice spleen, but kept getting poor yield. I found from literature that the frequency of FDC is around...

17 February 2021 3,506 4 View

Fluorophore panel selection?

Hello, I'm trying to select fluorophores for a large panel of flow cytometry, so I'm wondering what is the maximum degree of overlap between two fluorophores that is considered acceptable? Thanks.

19 January 2021 3,475 3 View

Will TNF alpha receptor 1/2 (TNFR1 or TNFR2) be up- or down-regulated when endothelial cells are exposed to TNF alpha?

Excessive exposure to TNF alpha leads to inflammation, oxidative stress, and other pathological responses in cells like endothelial cells. When cells are treated with TNF alpha, will the receptors...

21 November 2020 4,039 3 View

About mechanical design?

Hello, I am a graduate student majoring in mechanical design. My mentor doesn't have a project now, I' m confused.Could you tell me what project everyone is studying? The scope is probably product...

08 October 2020 5,464 3 View

How to import files generated by Neper into ABAQUS for analysis?

I used Neper to generate the tessellation file and the meshing file, and assigned the crystal orientation. How to import these files into ABAQUS for crystal plastic finite element analysis(CPFEA,...

03 February 2020 7,608 8 View

Looking for a type of non-dividing primary cells to culture?

Dear community, Happy new year! I have a very rudimental question to bother you with. I'm interested in the principles of epigenetics. All the cells I'm familiar with are in cell cycle, such as...

31 December 2019 1,991 2 View

Is it possible to access a node or element set in a abaqus subroutine?

We define a set in abaqus preprocessor and we want to access it in a abaqus subroutine. Is this possible? What should I do?

29 November 2019 9,027 1 View

How to get the SNP flanking sequence(for Prunus mume, Japanese apricot)?

I already get SNP candidates for our target traits, then I would like use Real-time PCR based genotyping platform to check F1 population. For establishing the marker, the SNP flanking sequences...

09 November 2019 331 3 View

'Unable to start analysis job' error in ABAQUS CAE with a subroutine?

When I try to start a simulation in ABAQUS CAE with *.inp and *.f(umat file). The abaqus just prompt an error:"Unable to start analysis job" and stop without any debug message. The example files...

24 October 2019 1,436 2 View

Problems about hourglass energy control and unreal element deformation in LS-DYNA impact analysis?

Hello, everyone, I want to ask a question which bothered me for a long time. I am conducting a verification analysis by LS-DYNA according to an RC columns impact experiment finished by Prof....

15 October 2019 5,812 3 View

Can Ethovision store the generated data and video recordings directly to NAS system?

The size of data generated by Ethovision is huge. Having NAS system for data storage seems to be a good option, I am wondering whether Ethovision can directly store the data to the NAS system...

27 February 2021 6,350 2 View

What are damage parameters for SMA while modelling on ABAQUS 2020?

I have given Elastic and Superelastic material properties, but I think we need to provide the damage parameters also. Please help me here.

16 February 2021 8,694 1 View

Using SILVACO to design tandem organic solar cells?

Hi Can anyone please suggest me how can I study the parameters of solar cells made of graphene oxide and any other organic material composite using SILVACO. I would also like to know how I can add...

15 February 2021 5,795 3 View

How to estimate interrater-reliability of a variable with 3 columns of answer?

Hello everyone, In my study, I asked participants to write 3 different memories. After collecting data, 2 raters coded these memories. Now I have 3 columns for the first rater and 3 columns for...

06 February 2021 7,463 8 View

What is the appropriate role of memorization in foreign language teaching?

Research in FLT has always been concerned with the role of memory in learning and the relationship between memorization and learning.

04 February 2021 2,202 5 View

Help me conduct a neuroscience experiment for my master's thesis?

22 January 2021 9,994 5 View

Which neuropsychological tests assess immediate and delay(24-48hrs) declarative memory/learning (verbal and visual) and procedural memory in children?

it can be either a neuropsychological assessmentfrom age 8 years old or a wel research paradigm

13 January 2021 2,308 2 View

What is the need of having weights at the output layer of an RNN ?

In RNN, Yt is the output which is given by the equation; Yt = Why.ht where Why is the weight associated with the output layer and ht is the current state. What is the need of having this weight...

11 January 2021 8,710 4 View

How to interact with internal memory of FPGA and connect it a signal or array inside a module?

I am trying to implement a processor tile on FPGA using vivado but it is taking too long to synthesize design due to large amount of Instruction and Data Memory. Currently, I have put complete...

05 January 2021 8,349 1 View

What is the best method to add nanoparticles in organic solar cell simulation using silvaco software ??

i built the structure of the organic solar cell (ito-P3HT_PCPM-PEDOT_PSS-al) then i used BEAM statement to add sunlight so what is the best method to add nanoparticles in layers

03 January 2021 7,107 11 View