Further we want to sequence the extracted RNA. So is there any major reason to choose mononuclear cells or white blood cells?
Hi, For example: If I have 20 eligible studies (Both case-control studies and cross-sectional studies without any control group ) coming out of a systematic search. Can I do meta-analysis with 10...
02 March 2019 4,562 7 View
I have been using RevMan for conducting meta-analysis. Recently I came to know about another software called "Comprehensive meta-analysis (CMA)". Anyone has experience of using both? Is there...
09 October 2016 6,110 13 View
Recently I came across a published (2006) article (Attached) which has been retracted in 2011. What might be the reasons of this retraction? Can the article be cited even after being retracted?
02 March 2016 6,854 1 View
According to the last diagnostic criteria of antiphospholipid syndrome (APS) (Miyakis 2006), anticardiolipin antibody (aCL) has to be > 40 GPL (IgG) or > 40 MPL (IgM). However, most of the...
02 March 2016 4,913 5 View
Sometimes researchers say above 2.0 impact factor is considered high! I wonder is there any such rule?
01 February 2016 359 1 View
When a journal's title changes, in ISI web of knowledge, I can see both impact factor against the previous journal title as well as the new title. For example BioMed Research International. The...
10 November 2015 1,980 3 View
Could anyone tell us whether there is any ranking of "indexing" besides ISI? Some say Scopus is the highest status as non-ISI indexed, some say Pubmed or so on. If there is any authentic website...
06 July 2015 2,174 2 View
06 July 2015 8,635 2 View
Sometimes it takes too long time for the journals to come up with a rejection (I have highest experience of 8 months). On the other hand, we are not encouraged or (it's possibly prohibited) to...
04 May 2015 2,898 18 View
Even after junior scientist's contribution in preparing a manuscript (requested by the senior scientist), if the senior scientist (corresponding author) excludes his name intentionally from a...
04 May 2015 6,467 1 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...
10 August 2024 8,539 5 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View