Electrochemical microrna biosensor
Hi Zohreh,
I believe that there is no difference at which end the modification will be made, as long as it does not interfere in hybridization with the target DNA.
Hello, I have a script changing the distances of two bonded atoms and I calculated the total energy (or potenial energy, kinetic energy is zero) with energy minimisation tool of lammps (ReaxFF...
27 March 2024 4,468 2 View
hi every one it is my first time I want to send cells for flowcytometry. I want to be sure is it okay to seed cells in 6 well plate for both apaptotic assay and cell cycle assay just in one...
26 January 2024 4,836 0 View
transfection, lipofectamine 2000
14 November 2023 2,379 4 View
Mtt assay
20 May 2023 709 2 View
The resulting film became brittle or two-phases.
01 February 2023 401 3 View
I'm currently exploring the gap between real and online space in urban tourism using the Python programming language. This gap usually contains unattractive facts and routines for tourists that...
10 May 2022 9,470 6 View
Is it possible to perform tension-compression fatigue test on very thin metallic samples? Can anyone suggest a paper/thesis? thanks
18 April 2022 7,364 4 View
In order to analyze the principal components (PCA) in the RStudio software environment, I installed various packages, including "ggplot2" , "factoextra" as well as "ggfortify". However, the...
23 January 2022 1,854 5 View
In general for compression tests, the D/L ratio should be less than 2, does this applies to pipe and tubes also?
29 June 2021 6,862 8 View
Hello and good day For the subject of my dissertation, I intend to study the issue of recovery of plant species after fire (Rim Fire). In this regard, I need educational data in this area, ie the...
26 April 2021 136 0 View
How to design VN primer to attach with universal reverse primer
05 August 2024 2,116 3 View
Hello everyone, I'm encountering an issue with my electrochemical impedance spectroscopy (EIS) measurements and would appreciate some insights. Experimental Setup: Electrodes: Gold interdigitated...
05 August 2024 3,783 2 View
I am trying to drop cast 2D Materials on Glassy Carbon Electrode for Cyclic voltametry and EIS purpose. What would be best binders to use?
23 July 2024 5,776 2 View
I work with planar interdigitated electrodes (IDEs). The finger width and the spacing between the fingers are 5 micron. The surface of the IDEs is coated by a non-conductive polymer layer...
16 July 2024 5,031 1 View
Hi, I am doing aptamer based electrochemical biosensor. First, I cleaned screen printed gold electrode using 0.5M H2SO4. Then, immobilized aptamer followed by blocking using 2mM MCH. I use...
06 July 2024 9,852 1 View
I am simulating 1 cell of PEM with an area of 50 cm². The mesh has an orthogonality of 0.2 for the channels and bipoar plates (polyhedra) and a quality of 1 for the active zones (hexagonal). The...
26 June 2024 2,358 0 View
Hello everyone, I am currently working on miRNA- mRNA interaction study. Further, I would like to do MD simulations for the miRNA-mRNA models. For the Simulation, I have modelled this complex...
24 June 2024 3,507 2 View
Hello to everyone, I'm trying to extrtact total RNA from sEVs. I made a first experiment starting from 2x10^10 sEVs quantified by NTA but the RNA obtained was very low (under 20ng/ml) Do you have...
18 June 2024 2,799 4 View
Recently, I have been trying to quantify the expression of miRNA. I have extracted the miRNA. However, I am not clear about using the cDNA synthesis kit usually there are two kits providing by...
16 June 2024 9,723 2 View
Hello everyone, I would like to know if any of you ever tried to assess the yeld of RNA extraction with the mirVana™ miRNA Isolation Kit, without phenol from ThermoFisher. I am experincing some...
06 June 2024 5,715 4 View