For immunocytochemistry, my primary antibody dilution is 1:4000, and i dont need that much of primary, as i only would use 500uls not 4000uls. how?

how can i use less? can i dilute the antibody in pbsX1 and keep using the diluted aliquot?

05 July 2018 2,536 3 View

I have undifferentiated dental pulp cells i want to stain with anti-NLRP3 antibody, how can i know the right dilution?

I am culturing undifferentitaed dental pulp cells and i want to stain for NLRP3 in the cell, i have the Millipore anti-NLRP3 antibody, it says on the site that they use 1:4000 antibody dilution...

24 May 2018 7,463 3 View

I have undifferentiated dental pulp cells i want to stain with anti-NLRP3 antibody, how can i know the right dilution?

I am culturing undifferentitaed dental pulp cells and i want to stain for NLRP3 in the cell, i have the Millipore anti-NLRP3 antibody, it says on the site that they use 1:4000 antibody dilution...

04 May 2018 4,418 3 View

Review paper about pharmacogenetic diabetes mellitus ???

09 October 2015 4,548 0 View

Are you attending the April Conference in Egypt?

Hello Dr Abdel-Aleem, Are you participating in the EVPA conference in Egypt at Porto Maria The North Cost of Egypt?? The Conference is from 20-23 April, 2019. Best Regards Al-Natour

01 January 1970 4,421 5 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

Will a 10xHis-tag prevent anti-MBP from binding to MBP?

I have a protein with a 10xHis-MBP tag on the N-terminus. I'm fairly certain I am expressing my protein, but I'm not getting a signal on my western blot. Is there a possibility that the 10xHis tag...

25 February 2021 8,404 3 View