series dilution 1:100, then 1:4000

Make a dilution according to your requirement and use it accordingly.

Dear Banan.

As Xinyan recommended you can make serial dilutions.

First dilute your AB 1:100 in PBS. You can use this aliquot for other stainings.

= 1ul AB + 99 ul PBS

Then dilute it 1:40 to get a 1:4000 dilution at the end.

= 12,5 ul of your 1:100 dilution + 487,5 ul PBS

Greetings