Is that possible to do an impedance measurement without FRA module?

One of my colleagues said, "the impedance you can measure with the three electrodes and with the specific sequence varying the frequency" Is it possible to configure the potenciostat without the...

21 February 2021 418 2 View

How to prevent cancer spheroid aggregation in culture?

I'm trying to grow PDX-derived cells from a SCCOHT model and I need them to survive short-term (7-14days) ex vivo After tumor digestion I platted 7,000,000 cells in a 10cm ULA dish In the...

26 February 2020 1,401 4 View

I made a saline solution of NaCl 0,9% andajusted the pH to 11 with NaOH. After 3 months I read the Ph and it had come down to 8. Does anyone know why?

I've also made a saline solution of NaCl 0,9% pH 9 and after 3 months it came down to pH 5. I don't understand why the pH came so drastcally down. I tested for any contamination and the results...

17 December 2018 8,793 3 View

I would like to talk to someone who extracts RNA from ganglion optics in crustaceans?

I'm trying to extract the RNA from the shrimp optical ganglion.

18 October 2018 2,959 1 View

Can you help me understand an inconsistency between copy-number variation of a gene and its expression?

Hi fellow scientists, I am researching a gene in Enterococcus faecalis, classified in two groups: 1) Penicillin-sensitive/ampicillin-sensitive (Control) and; 2)...

17 September 2018 9,809 4 View

Why the current backward is higher than the forward?

Hi, does anyone knows why my CV in KOH 1M and EtOH 1M of Palladium in graphene oxide the current forward is lower than the current backward? It should be the opposit, or am i wrong? In the...

05 April 2018 1,687 1 View

Cyclic voltametry of graphene oxide in a Ultra-thin layer electrode?

11 December 2016 8,898 3 View

How can I disperse hydrophobic particles onto glassy carbon surface?

Dear all, does anybody have an experience of carbon ink preparation which consists of hydrophobic nanoparticles dispersed into water-IPA mixture and a small amount of Nafion solution? I tried...

21 November 2016 8,312 3 View

Which method is better to determine the leakage of ionic liquids from polymer membranes?

Which method do you usually use to determine the degree of ionic liquids leakege from a polymer membrane? In term of Fuel Cell application. Thank you in advance!

13 June 2016 801 3 View

What is the proportion of T lymphocytes and of the other CD4+ cells among CD4+ PBMC of healthy humans? Is it altered in autoimmune diseases?

The CD4 molecule is expressed not only in T lymphocytes, but also in NKT cells, B cells, monocytes, and granulocytes (+ other?).

28 October 2012 7,741 6 View

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

Bought HUVEC from Invitrgen.need to revive it.expected it is cryofreezed in DMSO.Do I need to remove the DMSO for plating the cell for the 1st time?

After I cryofreeze the cell do I need to remove the DMSO for culturing the cell for next passages .

28 February 2021 7,738 3 View

POlymer+DMSO solution and moisture??

What could be the reason of moisture at the sides of Vial when I am preparing my polmyer+DMSO solution for membrane fabrication? What etiquettes I am possibly missing here?

28 February 2021 6,516 5 View

Diltuions for a Western blot?

I need help using the equation C1V1=C2V2 figuring out how much antibody to add to use for a western blot. The stock was origianlly 3.45 ng/ul but diluted 1:10 so now its 0.345 ng/ul. I need to run...

22 February 2021 9,901 3 View

How to dilute Toxin-DMSO solution?

I have a concentrated solution of a toxin(50 micromolar) in DMSO 100%. I need to dilute it inorder to dilute both the toxin and DMSO wich can be toxic to cells. Which would you guys recommend to...

19 February 2021 7,447 2 View

How to successfully revive cells stored at -80 degree C for a year?

I am working on C6 cells along with other neuronal cell lines. We have preserved the cells in 90%FBS and 10% DMSO solution. We are reviving the cells after a year and cells are either not binding...

18 February 2021 4,499 1 View

What are alternatives for B27+ Supplement in neuronal cell culture?

Hello everybody In our iPSC-derived neuronal culture we use Maturationmedium, supplemented with B27+. As Thermo Fisher run out of stock and can deliver earliest in May, I wanted to ask whether...

16 February 2021 3,348 1 View

How can I make sure that BCTC doesn't fall out of solution/form DMSO crystals when treating cells?

I resuspended my BCTC as described on the bottle at 10mg/mL in DMSO. However, I can see the formation of crystals which I can only assume are DMSO and that the drug is precipitating out of...

14 February 2021 5,633 1 View

Can anyone suggest a way to dissolve insulin which is for rat/mouse injection?

We are trying to inject insulin to rat or mouse, but the insulin we've got is powder, and there are many ways for dissolve, like in saline, PBS, DMSO, or in Tris-HCl, can anyone give us a...

13 February 2021 8,426 10 View

DMSO concentration in cell culture? Precipitating while PBS is added?

I am testing a compound on RAW 264.7. the compound is soluble in DMSO. but to ensure that final concentration of dmso is not more than 0.1%, we are first dissolving it in dmso and then diluting it...

11 February 2021 1,136 3 View