Is that possible to do an impedance measurement without FRA module?

One of my colleagues said, "the impedance you can measure with the three electrodes and with the specific sequence varying the frequency" Is it possible to configure the potenciostat without the...

21 February 2021 418 2 View

How to prevent cancer spheroid aggregation in culture?

I'm trying to grow PDX-derived cells from a SCCOHT model and I need them to survive short-term (7-14days) ex vivo After tumor digestion I platted 7,000,000 cells in a 10cm ULA dish In the...

26 February 2020 1,401 4 View

I made a saline solution of NaCl 0,9% andajusted the pH to 11 with NaOH. After 3 months I read the Ph and it had come down to 8. Does anyone know why?

I've also made a saline solution of NaCl 0,9% pH 9 and after 3 months it came down to pH 5. I don't understand why the pH came so drastcally down. I tested for any contamination and the results...

17 December 2018 8,793 3 View

I would like to talk to someone who extracts RNA from ganglion optics in crustaceans?

I'm trying to extract the RNA from the shrimp optical ganglion.

18 October 2018 2,959 1 View

Can you help me understand an inconsistency between copy-number variation of a gene and its expression?

Hi fellow scientists, I am researching a gene in Enterococcus faecalis, classified in two groups: 1) Penicillin-sensitive/ampicillin-sensitive (Control) and; 2)...

17 September 2018 9,809 4 View

Why the current backward is higher than the forward?

Hi, does anyone knows why my CV in KOH 1M and EtOH 1M of Palladium in graphene oxide the current forward is lower than the current backward? It should be the opposit, or am i wrong? In the...

05 April 2018 1,687 1 View

Cyclic voltametry of graphene oxide in a Ultra-thin layer electrode?

11 December 2016 8,898 3 View

How can I disperse hydrophobic particles onto glassy carbon surface?

Dear all, does anybody have an experience of carbon ink preparation which consists of hydrophobic nanoparticles dispersed into water-IPA mixture and a small amount of Nafion solution? I tried...

21 November 2016 8,312 3 View

Which method is better to determine the leakage of ionic liquids from polymer membranes?

Which method do you usually use to determine the degree of ionic liquids leakege from a polymer membrane? In term of Fuel Cell application. Thank you in advance!

13 June 2016 801 3 View

What is the proportion of T lymphocytes and of the other CD4+ cells among CD4+ PBMC of healthy humans? Is it altered in autoimmune diseases?

The CD4 molecule is expressed not only in T lymphocytes, but also in NKT cells, B cells, monocytes, and granulocytes (+ other?).

28 October 2012 7,741 6 View

Can I perform two way ANOVA in case of some missing groups?

I had control cells and performer ELISA analyses group (time 0). I stimulated cells with substance A and B and cultivated for 1, 2 and 3 hours. On each cells I performed ELISA. I have results...

17 February 2021 8,933 4 View

Any probe or assay for the detection of Cysteine in yeast invivo ?

Dear fellow researchers, I would like to detect the cysteine levels for yeast in vivo. I would really appreciate if anyone could suggest me any fluorescent probe or assay kit available. Currently...

11 February 2021 3,292 3 View

Which fungicide are good for primary cells? Does it yeast contamination? How to get rid of them without killing the cells? How to get rid of spores?

Hello! Our lab is working with primary cells which we are isolating from the colorectal tissue. During the isolation, we are washing the tissue with pen-strep and then also add some pen-strep in...

07 February 2021 8,258 3 View

What is the relationship that determines the depth of the mechanical cultivator with the age or size of the weed For crops planted in lines?

the great depth of the cultivator may lead to passage under the roots without killing them and may give negative results to break up the soil below the roots, increasing their activity, especially...

03 February 2021 3,198 7 View

How to Remove DNA From a Sample Without Rupturing Yeast?

I transformed a plasmid into yeast and selected for transformants by growing them in a selective media (liquid, not agar plate). My experiment requires that I continually grow/ split these...

01 February 2021 9,936 2 View

Western blot transfer problem - what could cause it?

Hi everyone, We are doing western blot with yeast protein extracts. We are applying a wet transfer method using Tris-Gly as a transfer buffer (with 10% methanol) and running it with constant...

25 January 2021 4,228 6 View

Can someone explain why my A260/A280 ratio is lower than 1?

We are conducting a measurement of Ribonucleic acid from baker's yeast (S. cerevisiae) (Sigma-Aldrich R6750) concentration using Eon Biotek Microplate reader. We dilute the RNA in various...

24 January 2021 9,830 3 View

Has someone used TTC agar overlay method and can help me?

I have tried to use TTC agar overlay method for a few times to identify respiratory-deficient yeast which were affected by EthBr. The problem is that cells which should have stayed white also have...

22 January 2021 585 10 View

Is MOPS buffer needed for in vitro horizontal gene transfer in thr coculture of two different pathogenic bacterial species?

Actually i am working on possibilities of horizontal gene transfer of resistant gene in broth coculture of two different types of bacteria, and the protocol i follow involves the use of MOPS...

19 January 2021 4,566 2 View

How can I insert antibody sequences into yeast cell to achieve stable and large quantity production?

I have the sequence of the light chain and heavy chain of a certain antibody. I would like to know how to express this antibody in yeast cells to achieve stable and large quantity production. It...

04 January 2021 9,669 2 View