Is that possible to do an impedance measurement without FRA module?

One of my colleagues said, "the impedance you can measure with the three electrodes and with the specific sequence varying the frequency" Is it possible to configure the potenciostat without the...

21 February 2021 418 2 View

How to prevent cancer spheroid aggregation in culture?

I'm trying to grow PDX-derived cells from a SCCOHT model and I need them to survive short-term (7-14days) ex vivo After tumor digestion I platted 7,000,000 cells in a 10cm ULA dish In the...

26 February 2020 1,401 4 View

I made a saline solution of NaCl 0,9% andajusted the pH to 11 with NaOH. After 3 months I read the Ph and it had come down to 8. Does anyone know why?

I've also made a saline solution of NaCl 0,9% pH 9 and after 3 months it came down to pH 5. I don't understand why the pH came so drastcally down. I tested for any contamination and the results...

17 December 2018 8,793 3 View

I would like to talk to someone who extracts RNA from ganglion optics in crustaceans?

I'm trying to extract the RNA from the shrimp optical ganglion.

18 October 2018 2,959 1 View

Can you help me understand an inconsistency between copy-number variation of a gene and its expression?

Hi fellow scientists, I am researching a gene in Enterococcus faecalis, classified in two groups: 1) Penicillin-sensitive/ampicillin-sensitive (Control) and; 2)...

17 September 2018 9,809 4 View

Why the current backward is higher than the forward?

Hi, does anyone knows why my CV in KOH 1M and EtOH 1M of Palladium in graphene oxide the current forward is lower than the current backward? It should be the opposit, or am i wrong? In the...

05 April 2018 1,687 1 View

Are my cells stained with Hoechst 33342 for mycoplasma test infected?

I'm cultivating 3T3-F442A murine pre-adipocytes for an experiment. They suddenly started growing slowly and showed abnormal morphology. I suspected of mycoplasm and as I don't have a kit for...

06 March 2018 8,096 7 View

Cyclic voltametry of graphene oxide in a Ultra-thin layer electrode?

11 December 2016 8,898 3 View

What is the proportion of T lymphocytes and of the other CD4+ cells among CD4+ PBMC of healthy humans? Is it altered in autoimmune diseases?

The CD4 molecule is expressed not only in T lymphocytes, but also in NKT cells, B cells, monocytes, and granulocytes (+ other?).

28 October 2012 7,741 6 View

How can you be so sure that your subjective experiences (material or not) actually exist in your brain? How would you describe it?

I mean, the brain tricks us in many different ways, why wouldn't it do the same regarding qualia (and many other things)?

01 January 1970 2,881 13 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

How to compare different concentrations of nanoparticles for characterization from ICP-MS?

I took measurements of different concentrations of 15 nm and 5 nm Au nano particle solutions. I'm trying to determine which concentration I measured gives the best reading overall. I also...

02 March 2021 7,626 1 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

IF troubleshooting: looking at an intracellular protein that localizes to the cell's and how to reduce increase in signal due to cell ruffles?

I am currently trying to stain for PI3KBeta in MDA-MB-231 cells. My current issue is that 1) beta stains all over the cell and 2) there is an increase in signal at the edge of the cell and this...

24 February 2021 8,582 1 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

Have you ever experiencing air vent trouble in fermentation process?

Dear all, My research group is currently working with a 12-litre fermenter. Somehow, if we do the fermentation for more than 20 hours, the process will be more prone to contamination. Our current...

23 February 2021 1,908 2 View

How to clean up TEM grid with plasma cleaner?

I need to clean the hydrocarbon contamination on the carbon substrate of TEM grid. However, I heard that substrate could be broken by the powerful plasma. My substrate is pure carbon, with ~3nm,...

23 February 2021 3,262 5 View

What are some underexplored theories and concepts in Activated Carbon as A Water Filter?

Hi, me and my team mates are currently planning to pursue the topic of Activated Carbon from Coconut as a Water Filter. And we would like to ask for your suggestions and knowledge on what specific...

22 February 2021 6,569 3 View

What are some underexplored theories and concepts in Activated Carbon as A Water Filter?

Hi, me and my team mates are currently planning to pursue the topic of Activated Carbon from Coconut as a Water Filter. And we would like to ask for your suggestions and knowledge on what specific...

21 February 2021 7,561 4 View

What properties of water shall be analyzed and evaluated to test the effectiveness of activated carbon?

I would like to ask what other properties of water (pH and such) shall be analyzed and evaluated to test the effectiveness of activated carbon from filtering the contaminants present in the water?

20 February 2021 9,422 8 View