Can Vicon data be used as input in online real-time experiment?

I wanted to know if the Vicon system, as a motion capture device, can be used for real-time processing data. Can we collect online data em then use it as input to activate other devices? During an...

13 November 2019 3,790 4 View

Can a SNP be considered an epigenetic variation?

I'm trying to understand epigenetic variability but the study I'm performing involves measuring the gene expression in a given region according to the SNP's alleles associated with the increase of...

22 June 2019 6,511 12 View

Does anyone have experience with chemokine receptor staining of NHP samples?

I have been staining NHP samples for flow cytometry analysis with CXCR5 and it seems that the CXCR5+ population is mostly lost when I stain with a panel that includes incubation with BD...

20 June 2019 4,961 1 View

How I can analyse the lipids, proteins and carbohydrates from estuarine sediment?

will be necessary analyse the nutritional quality of the sediment, if I analyse only the macrofauna?

05 June 2019 270 3 View

FFPE DNA/RNA extraction from FFPE samples. Can we frozen the cuts?

Hi everyone, I need to perform DNA/RNA extraction from FFPE samples. The kit that I started using for DNA specifically says that the cuts from the section need to be fresh but I was told that...

11 September 2018 6,194 3 View

I want to test if a protein binds directly to the plasma membrane using In vitro and microscopy. How can I do it?

21 October 2017 2,890 2 View

How to avoid endogenous acetylation of E. Coli?

I'm trying to use a protein purified in E. Coli for an experience, however the protein is acetylated during expression in E. Coli and it compromises my work. Is there any way to avoid the teh...

08 December 2016 2,999 4 View

Is it possible to use Tet-system when working with bacteria-infected cells?

Is possible to use this system when working with bacteria-infected cells that are susceptible to Dox, or it is better to use other system of gene expression induction? If so, do you know any?

06 July 2016 5,375 2 View

Nitrate Reductase assay in Rice

I've been reading several journal articles pertaining to Jaworski's (1971) nitrate reductase assay using intact plant tissues. may i know the specific method and reagents used in this assay? Is it...

03 March 2016 6,811 2 View

How to overcome the interference of color from plant extracts in alpha-glucosidase assays?

28 February 2016 154 4 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

In PRIMER, is it possible to downweight certain taxa in multivariate community datasets to reflect uncertainty around identification??

I have a dataset with about 80 different species. As usual, some species are very easy to identify with certainty whereas others are more difficult, which means that I am less certain of my...

03 March 2021 8,066 4 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

How to calculate the annealing temperature of a primer pair?

I have to amplify a gene and my primers just reached. The Tm for Forward primer is 64.2, and that of reverse primer is 65.5. Can some one suggest how to get the best annealing temperature? Thanks...

01 March 2021 360 7 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

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Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

Anybody with an idea on how do design specific primers for detecting tomato resistance genes tm-1, TM-2 and Tm2^2?

I am trying to identify these 3 genes among some tomato cultivar collections and after aligning some sequences from NCBI, I couldn't find unique sequences to target for specific primers. There...

28 February 2021 606 3 View