Joana Maclean

5 Questions 2 Answers 0 Followers

Questions related from Joana Maclean

Potential nitrification assay (Griess method) on soil stored at -20°C?

We are measuring soil nitrification potential in soil using the Griess-Ilosvay's method, according to Raglan et al., 2022 (see extract below). We have in total 360 soil samples which were stored...

14 August 2023 4,063 1 View

Looking for a protocol to wash and reuse plastic cuvettes?

"Reusing plastic cuvettes is not recommended" This is how far I got in my search for a safe and economic way to wash and reuse polystyrene cuvettes for measuring cell cultures OD's. I understand...

31 March 2022 8,059 1 View

Can someone explain how NILE Red intercalates with synthetic polymers like PE?

The fluorescent dye NILE Red had originally been used for staining of hydrophobic compounds like lipids. In the last years, NILE Red has been tested and proposed for the staining of microplastic...

28 August 2018 6,142 5 View

Alternatives for using HMDS in SEM sample preparation ?

Hello, I'm fixating bacterial biofilms on plastic surfaces for SEM imaging and I don't have the facilities for critical point drying, nor access to Hexamethyldisilizane (HMDS) as a drying...

23 August 2017 7,240 4 View

For gradient PCR, could I prepare a Master Mix with DNA template already added?

I'm optimizing primer pairs with gradient PCR and I have a lot of replicates, using the same DNA template. I was wondering, if I could prepare my Master Mix already with my DNA template added in...

01 January 1970 9,700 4 View