11 September 2023 1 5K Report

I have an idea on how to dissect motor cortex from mouse and I'd like to find an article or a book, does anyone have some piece of information?

More Joana Antonela Asensio's questions See All
Housekeeping genes and comparing data?

Hello everyone, I work with skin cells and am performing RT-qPCR of primary fibroblast cells and primary keratinocyte cells to further use as a comparison to iPSC-derived skin cells and 3D skin...

22 April 2024 6,181 2 View

How can we measure distances from points to a group centroid in GM?

I am new to Geometric Morphometrics, and I'm not sure this is possible or doable. I have 133 photos with 16 landmarks and 23 semilandmarks of two species and admixture individuals. Their group...

10 April 2024 3,829 2 View

Which methods are the most appropriate and reliable for evaluating antioxidant activity in extracts from medicinal plants?

Hello everyone, In my lab we are currently using the FRAP, ABTS, and DPPH assays to analyse antioxidant activity in medicinal plant extracts. However, we have recently received constructive...

05 March 2024 8,954 3 View

Cryopreservation of brain tissue?

Which method is better to prepare brain frozen sections? A) Directly, after sacrifice freeze as rapidly as possible at -80ºC without fixative. Fix before immunohistochemistry / IFI. B) Fix in 4%...

28 August 2023 8,874 4 View

How to perform a 4 parameter logistic equation for ELISA?

I have measured Irisin levels in plasma and now I'm trying to analyze the results. As far as I have read, I need to perform a 4 parameter logistic regression, should I use logarithm for...

21 August 2023 461 4 View

How to dilute serum samples for Irisin by ELISA?

I have to measure irisin levels in mouse serum samples and I would like to estimate the dilution of the sample, has anyone else done this before? The manufacturer recommends 1:8

15 August 2023 6,165 3 View

Potential nitrification assay (Griess method) on soil stored at -20°C?

We are measuring soil nitrification potential in soil using the Griess-Ilosvay's method, according to Raglan et al., 2022 (see extract below). We have in total 360 soil samples which were stored...

13 August 2023 4,110 1 View

Opinion on Journal Food Chemistry Advances?

What do you think about the Journal Food Chemistry Advances? Does it worth publishing in there?

24 July 2023 8,133 5 View

Can I add data with standard deviation in SPSS?

I have a dataset of results obtained from a technique of XRF. However, I only received the results with the mean and the variation of three replicas. Can I add the dataset with mean + standard...

17 May 2023 751 4 View

Ideal Fluo-4 AM concentration?

Hello, I am designing a calcium receptor assay and couldnt find an ideal concentration for Fluo-4 AM. Does anyone have any idea? Thank you in advance!

15 February 2023 9,190 1 View

Similar questions and discussions
Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Text-Communication from the M1 Hand Area using BCI—and then there is Elon Musk?

Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...

10 August 2024 7,180 0 View

Mouse CD3 antibody sequence?

Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much

08 August 2024 9,157 4 View

An animal in not a rock (e.g., Koch 2013), it has an internal constitution that can be modified?

Vertebrates and invertebrates (e.g., arthropods: insects, crustaceans, etc.) have two different neuro-muscular systems given their differential construction: the former have an endoskeleton and...

07 August 2024 648 0 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

06 August 2024 9,710 1 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

In what part of the brain´s rabbit bdnf is located and include references please?

I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....

04 August 2024 762 1 View

Tissue homogenization in mouse with FastPrep-24™ 5G - is the D matrix system useful?

I need to lyse and homogenise mouse tissues. So far I have experimented with liver and lung tissue, using matrix D. The liver was completely lysed with 6 repetitions of the standard program, but...

31 July 2024 9,186 0 View

How to Post-fix Mice pup's brains?

I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...

31 July 2024 1,123 4 View

Our partners will collect data and use cookies for ad personalization and measurement. Learn how we and our ad partner Google, collect and use data. Agree & close