Can Vicon data be used as input in online real-time experiment?

I wanted to know if the Vicon system, as a motion capture device, can be used for real-time processing data. Can we collect online data em then use it as input to activate other devices? During an...

13 November 2019 3,790 4 View

Can a SNP be considered an epigenetic variation?

I'm trying to understand epigenetic variability but the study I'm performing involves measuring the gene expression in a given region according to the SNP's alleles associated with the increase of...

22 June 2019 6,511 12 View

Does anyone have experience with chemokine receptor staining of NHP samples?

I have been staining NHP samples for flow cytometry analysis with CXCR5 and it seems that the CXCR5+ population is mostly lost when I stain with a panel that includes incubation with BD...

20 June 2019 4,961 1 View

How I can analyse the lipids, proteins and carbohydrates from estuarine sediment?

will be necessary analyse the nutritional quality of the sediment, if I analyse only the macrofauna?

05 June 2019 270 3 View

Does somebody know which dye can be used for total protein normalization in confocal microscopy?

Is there any fluorescent dye for total protein staining in confocal microscopy?

31 January 2019 3,501 0 View

FFPE DNA/RNA extraction from FFPE samples. Can we frozen the cuts?

Hi everyone, I need to perform DNA/RNA extraction from FFPE samples. The kit that I started using for DNA specifically says that the cuts from the section need to be fresh but I was told that...

11 September 2018 6,194 3 View

I want to test if a protein binds directly to the plasma membrane using In vitro and microscopy. How can I do it?

21 October 2017 2,890 2 View

How to avoid endogenous acetylation of E. Coli?

I'm trying to use a protein purified in E. Coli for an experience, however the protein is acetylated during expression in E. Coli and it compromises my work. Is there any way to avoid the teh...

08 December 2016 2,999 4 View

Is it possible to use Tet-system when working with bacteria-infected cells?

Is possible to use this system when working with bacteria-infected cells that are susceptible to Dox, or it is better to use other system of gene expression induction? If so, do you know any?

06 July 2016 5,375 2 View

What is the best flow cytometer to analyze microparticles?

In your opinion, what is the best flow cytometer that currently exists in the market to analyze microparticles? Thank you

15 June 2016 4,762 3 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

What are the pros and cons for mice versus rats?

Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...

24 February 2021 1,825 3 View

Is it harmful to breed for Cre Homozygosity initially?

Hello, I am currently using a Cas9 knock in system that will be expressed in the liver. To do so STOP codon fl/fl Cas9 KI mice were bred with Albumin-Cre mice to generate heterozgous pups, that...

24 February 2021 5,759 3 View

How to empty large intestine in mice ?

Hi all I am working on colon-targeted drug delivery for which I kept mice fasting for 6h using fasting grills during scotophase (dark period). I observed liquid fecal content in the cecum and...

23 February 2021 7,701 2 View

Does PHA-P stimulate the proliferation of lymphocytes from mice?

Hello, I will start performing MLR using lymphocytes from mice's spleen and I would like to know the better way for the stimulation. If is using PHA-P or CD3/CD28? I would like to know as well if...

23 February 2021 5,476 4 View

In frequency domain alpha band(8-16hz), beta band(16-32hz), gamma band(32-64hz), sub-band(64-128hz) are same as LL,LH,HL,HH in wavelet decomposition?

I am working on single trial motor imagery data i.e. EEG data. I am following a paper which has a dataset from BCI challenge IV (dataset 2b). After removing noise and extracting all the data from...

23 February 2021 5,503 1 View