Maynak Chakraborty

10 Questions 49 Answers 0 Followers

Questions related from Maynak Chakraborty

Why does SPSS show odd ratio of control/case instead of case/control?

I have done a genetic association analysis based on SPSS software. The allelic association has come out as a significant association. But when I wanted to compute the odds ratio in both allelic...

19 February 2022 5,897 7 View

Which statistical test is best for additive model of genotypic study?

I am planning to do an analysis of both allelic and genotypic level of association of an particular SNP with a disease via case control study.But, I can not understand which statistical test...

05 August 2019 3,560 3 View

What would be the consequences if I take equal ratio of renilla plasmid rather than equal amount in my promoter analysis through luciferase assay?

For my promoter activity analysis, I take 0.12pmol of each different size of construct and 1:100 ratio of renilla vector. I balance the amount of total vector using a filler plasmid. 1) Construct...

10 March 2019 2,022 2 View

Which transcription start site of the gene should I choose for promoter analysis?

My experimental gene has 5 different transcription start sites.From different web based tools, I searched that the predominance of these sites in different tissues but still now I have not get any...

26 March 2017 3,729 5 View

Could anyone please help me to develop a cell culture based model system for Fuchs Dystrophy?

I am interested to understand the genetic pathophysiology of Fuchs Dystrophy and for that purpose it is required a cell culture based model system of Fuchs corneal endothelium cell line. We have...

12 March 2017 7,612 3 View

How can I calculate cell seeding number for 6-well and 24-well plates?

From my previous experiment, I have calculated X cells/ml and Y number of total cells from a T-25 flask.Like others, first I trypsinized the cells from T-25 flask, counted in a haemocytometer and...

04 February 2017 3,542 4 View

How can I calculate how many cells should I seed for my cell culture experiment ?

In general from a confluent flask first I trypsinize and pellet down the cells, re suspend in 2 ml of media.Then I take 100ul of cell suspension and add 4 volume of trypan blue.After 5 mins, the...

11 January 2017 6,827 4 View

Can I do competent cell preparation outside the hood ?

I have done competent cell preparation for cloning ( DH5 alpha) by Rubidium chloride method first time.But, by mistake the entire experiment I have accomplished outside the hood.Is it okay or I...

10 November 2016 8,584 6 View

What is the best vector for my luciferase assay ?

I want to know whether transcription factor X binds to promoter region of my gene or not through luciferase assay and like to prefer a vector which has minimal promoter plus MCS site upstream of...

20 October 2016 4,568 5 View

How can I create a knock-down,stable cell line without using viral based vectors?

I want to create a knock-down, stable cell line using crispr-cas system without using viral based vectors. I want to regulate the knock-down intensity around 60% so that I can do my experiments...

21 September 2016 8,900 3 View