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Questions related from Maynak Chakraborty
I have done a genetic association analysis based on SPSS software. The allelic association has come out as a significant association. But when I wanted to compute the odds ratio in both allelic...
19 February 2022 5,983 7 View
I am planning to do an analysis of both allelic and genotypic level of association of an particular SNP with a disease via case control study.But, I can not understand which statistical test...
05 August 2019 3,621 3 View
For my promoter activity analysis, I take 0.12pmol of each different size of construct and 1:100 ratio of renilla vector. I balance the amount of total vector using a filler plasmid. 1) Construct...
10 March 2019 2,087 2 View
I am planning to do a CRISPR-Cas9 HDR based knock-in into the exonic region of my gene. Generally, it is well suggested that, for knock-in, using ssDNA as a donor template is very efficient rather...
04 January 2018 4,137 1 View
I am a beginner of cDNA cloning for overexpression of my protein in cell line. I have extracted mRNA from cells, converted it into cDNA using Quantitect reverse transcription kit (Qiagen) and...
07 July 2017 9,929 3 View
For my experimental purpose ,I have to do mass spectrometry to analyse the expression of different proteins, derived from cell lines. I have decided that Spike- in SILAC is best for me.For SILAC...
16 April 2017 9,219 9 View
11 April 2017 4,539 4 View
I am a beginner of SILAC based mass spectrometry and I want to know the differential protein expression both my control and experimental cell lines. From various literature studies I think that...
07 April 2017 7,028 4 View
My experimental gene has 5 different transcription start sites.From different web based tools, I searched that the predominance of these sites in different tissues but still now I have not get any...
26 March 2017 3,816 5 View
For some experimental purposes, I have to culture primary corneal endothelial cells. But, in different literatures,the protocol of the culture seems different to me and for that now I am a little...
15 March 2017 3,746 5 View
I am interested to understand the genetic pathophysiology of Fuchs Dystrophy and for that purpose it is required a cell culture based model system of Fuchs corneal endothelium cell line. We have...
12 March 2017 7,684 3 View
For promoter assay I have chopped my promoter into six different segments and cloned each one into pGL3 basic vector for luciferase assay to analyse the activity of each segment. I co-transfect...
07 March 2017 4,425 2 View
From my previous experiment, I have calculated X cells/ml and Y number of total cells from a T-25 flask.Like others, first I trypsinized the cells from T-25 flask, counted in a haemocytometer and...
04 February 2017 3,635 4 View
In general from a confluent flask first I trypsinize and pellet down the cells, re suspend in 2 ml of media.Then I take 100ul of cell suspension and add 4 volume of trypan blue.After 5 mins, the...
11 January 2017 6,971 4 View
I have done competent cell preparation for cloning ( DH5 alpha) by Rubidium chloride method first time.But, by mistake the entire experiment I have accomplished outside the hood.Is it okay or I...
10 November 2016 8,665 6 View
My gene X has several transcript variants. Through UCSC genome Browser, I found the promoter sequence of each variant.But now I am a little bit confused because I do not know among all of those...
22 October 2016 5,669 2 View
I want to know whether transcription factor X binds to promoter region of my gene or not through luciferase assay and like to prefer a vector which has minimal promoter plus MCS site upstream of...
20 October 2016 4,645 5 View
I want to create a knock-down, stable cell line using crispr-cas system without using viral based vectors. I want to regulate the knock-down intensity around 60% so that I can do my experiments...
21 September 2016 8,966 3 View
