Fold change calculation?

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How to empty large intestine in mice ?

Hi all I am working on colon-targeted drug delivery for which I kept mice fasting for 6h using fasting grills during scotophase (dark period). I observed liquid fecal content in the cecum and...

23 February 2021 7,701 2 View

Can I express protein of plant origin in E. coli strain (M15) using pET28a vector systems? Are M15 strains and pET vector systems compatible?

Are E.coli strains M15 and pET28a vector systems compatible? Can I over-express my protein which is of plant origin. Previously I was using BL21 DE3 strains. Was confused to use M15 strains for...

10 February 2021 2,564 3 View

Could not get reduction peak for Mxene Counter electrode in Iodide electrolyte, why?

I have deposited MXene Ti2C2Tx mixed with tritonX binder and heated at 250 C for half an hour in ambient air conditions on FTO glass substrate, the deposition technique followed was doctor...

28 November 2020 2,330 6 View

What are the best binder which can be used for deposition of MXene on FTO?

I would like to coat MXene (Ti3C2Tx) on FTO glass substrate using doctor blade deposition technique, for which i think a binder is essential for the adhesion of MXene onto the FTO. The post...

28 November 2020 9,360 3 View

Kindly suggest the best organic solvents used for dispersibility of graphite powder?

Kindly suggest me some good papers regarding a comparative study on various organic solvents used as dispersion medium for graphite powder. The application is to make a graphite paste and used the...

24 October 2020 1,586 4 View

What are the best used slurry to coat on TCO using doctor blade method?

I'm trying to deposit TiO2 onto FTO beforehand I would also like to have some knowledge on the other different types of slurry consisting of different materials to coat on TCO using doctor blade...

22 October 2020 1,427 1 View

Could anyone please suggest me some papers on using MXenes (Ti3C2Tx) as CE for DSSC application?

I'm currently working on MXenes as Counter electrode (CE) for Dye sensitized solar cell (DSSC) application. I would like to know about the research work being done in this area. Thank you in advance.

14 October 2020 9,448 2 View

Is it possible to coat/mix MXene (Ti3C2Tx) with conductive polymers such as PEDOT or PANI?

Could anyone suggest me some good papers which involves the coating or mixing of MXenes with conductive polymers such as PEDOT or PANI or any other conductive polymer for that matter.

12 October 2020 5,653 3 View

There is an ambiguity in understaanding stability from Bode plot. Could you help me out with this?

T1 = -67.34 s + 1.563e06 ---------------------------------------------------------- 0.0008762 s^2 + 0.09793 s + 2273 T2 = 2.447e07 s^2 + 7.695e11 s + 2.687e13...

05 October 2020 6,138 7 View

What are the various methods by which MXenes are deposited on the Transparent conducting oxide glass?

I would like to know the different deposition techniques and the respective conditions and constraints to deposit or coat a layer of MXene (Ti3C2Tx) onto a TCO glass substrate.

04 October 2020 4,616 3 View

How to compare different concentrations of nanoparticles for characterization from ICP-MS?

I took measurements of different concentrations of 15 nm and 5 nm Au nano particle solutions. I'm trying to determine which concentration I measured gives the best reading overall. I also...

02 March 2021 7,626 1 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

I want to performe MALDI-TOF MS for bacterial identification, I would appreciate it if anyone can tell me the matrix solution preparation protocol ?

based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...

26 February 2021 6,799 3 View

How to make a time series imputation benchmark ?

Hello ! In a work, I have to benchmark different algorithms to fill in missing values in time series. I insist on the fact that this is imputation and not forecasting. In my case, I have access...

24 February 2021 7,470 7 View

How does the injection volume impact LLOQ and ULOQ in regression analysis (LC-MSMS)?

Hello, I have run into an issue that has sparkled a debate at work: is the LLOQ/ULOQ impacted by injection volume under the following circumstances: 1) Same dilution factor of all samples 2)...

23 February 2021 9,159 3 View

Why ICP-MS test for Teflon filters that collected PM2.5, show really high results in the millions of ug/g ?

I analyzed soil and ash samples using ICP-MS for 28 metals, then I resuspended those samples to collect PM2.5 particles in a filter using a selective inlet inlet. My only issue that Fe and Al...

13 February 2021 7,592 3 View

What influences inter-day reproducibility in UPLC-MS/MS analysis?

I was analyzing some metabolites in human plasma. While the intraday precision of these metabolites was acceptable ( less than 20%), the interday precision was quite large. So what influences...

13 February 2021 9,183 9 View

How do I prepare and store insect samples for JH measurements?

I need to preserve termite samples for future quantification of juvenile hormone in their bodies. Or extract the Juvenile Hormone and keep that samples stored for future quantification. I may need...

09 February 2021 1,995 1 View

MS/MS protein analysis?

Hi Everyone: I want to quantify the relative amount of a protein in 2 samples. I will first digest the sample with trypsin, and subject the resulting peptides to LC-MS/MS analysis. I will then...

25 January 2021 8,333 3 View

2.6 Drying gas flow controller cannot regulate the requested setting after a fixed amount of time?

We keep getting this error on our Agilent 6470 TQ. We were told it could either be our N2 generator or filter traps. We've called the manufacturer of our generator and they believe it's working...

21 January 2021 2,969 1 View