What is a standard read out or assay to analyze the effect of dosages of Mitomycin C on cell lines? How is dosage standardization done for any cells?

I need to perform scratch assay to study migration of cancer cells upon various treatments of my molecule of interest. However, to block proliferation using mitomycin C, the dosage would vary for...

21 January 2018 4,565 1 View

How to plot fraction of the cell recovered v/s concentration of the drug ?

07 August 2017 619 1 View

How to Identify the interacting partner from whole cell protein ?

The gene X has been taken from host namely Z, cloned and expressed X in E.coli. I would like to understand the interaction partners of X protein in host A. As X is his-tagged protein, purified and...

04 May 2017 8,808 6 View

How to attain the desired dilution factor for particular CFU ?

Hello , I 'm having the total count of 1.75*10^13 CFU/mL of bacteria. For the assay, I need 5*10^5 to 1*10^6 CFU/mL. To understand the total dilution factor which I shall use for attaining the...

09 October 2016 6,182 6 View

Can anybody help me out with choosing the appropriate statistical test for analyzing my data ?

I have zebrafish embryo samples - control vs drug treated and I analyze the phenotype outcome of the drug treatment on certain neuronal populations (qualitative analysis). I have 15 fish embryos...

07 September 2016 8,121 8 View

Can I use Dasatinib for a short term TCR blocking functional assay?

I want to check for TCR mediated responses and when I use a TCR blocking antibody in my 9 hour assay it results in activation (my bad! ) so I was wondering if using dasatinib could be any good to...

24 February 2016 3,192 1 View

Why can I not see bands in my agarose gel after running my PCR product of sediment DNA?

crude DNA was1:10 diluted and used as template for PCR in a 50uL mixture and 4uL of the amplified product was mixed with 1uL of 6x gel loading buffer and run in a 0.8% agarose gel using 1x TE...

02 June 2015 1,304 10 View

How can one determine the ori site of plasmid is compatible with the bacteria of interest or not?

03 April 2015 6,355 5 View

Is it difficult to isolate membrane proteins from gram positive bacteira. and how the protocol will be very different from negative to positive

07 August 2014 6,813 1 View

What might be causing no amplification in PCR?

07 August 2014 6,877 21 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

Can chitosan alone can act as a reducing agent for Cu(0) nanoparticle sythesis?

[Synthesizing Cu nanoparticles without using chemical reductants]

01 March 2021 8,359 1 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

I want to performe MALDI-TOF MS for bacterial identification, I would appreciate it if anyone can tell me the matrix solution preparation protocol ?

based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...

26 February 2021 6,799 3 View

Can I use acetic acid with an ELITE WAX Perkin Elmer column for Gas Chromatography?

Hi! I would want to know if I can use acetic acid with an ELITE WAX Perkin Elmer column for Gas Chromatography. This column has a polyethylene glycol phase, but I can't find the manual to check...

22 February 2021 730 2 View

Can you perform the Murexide test on samples fixed in Formalin?

I have a fixed sample and would like to use the murexide reaction to test for uric acid composition. I no longer have fresh samples

22 February 2021 1,328 1 View

Is there an ideal incubation time of RGO-modified SPCE before subsequent use, i.e., electrochemical characterization or further modification?

I notice that upon washing and air-drying of my RGO/SPCE surface and characterizing again via DPV, the current response towards ferricyanide probe significantly decreases. My worry is that the...

22 February 2021 5,324 3 View

What is the best etching method for Mg-Ca alloy?

I have used Picral etchant (4.2 g Picric acid, 10 mL Acetic acid, 70 mL Ethanol, 10 mL distilled water) but it didn't work. Is there any alternative?

21 February 2021 2,558 5 View

Protocol for membrane extraction E. coli?

Hello! I am interested in determining whether a poly-unsaturated fatty acid I have engineered E. coli to produce is being incorporated into the membrane of the cells or if it is simply a free...

21 February 2021 7,447 2 View

What is the stability of 2 3-dihydroxyquinoxaline in acidic water?

I'm developing a UHPLC assay, and the fluorescent product is 2,3-dihydroxyquinoxaline. The reaction to produce this requires hydrochloric acid (about 1.2 M). Will storage in this acidic...

20 February 2021 5,820 4 View