Could not amplify CD28 and CD3z ectodomain from cDNA of Jurkat Cells ?

For my experiments, I have to express ectodomains of human CD28 and CD3z. As a source, I extracted RNA from Jurkat cells and verified the quality of RNA on the gel. I, then, prepared cDNA using...

23 February 2021 7,051 4 View

Is it possible for Cre positive mice to be negative for flox reporter gene (eGFP)?

I was genotyping some mice tail snips. It is a Cre Lox reporter mouse. The Cre is expressed using CRH promoter in the mouse brain and eGFP is the reporter gene. I isolated g-DNA and used it for...

12 January 2021 6,875 3 View

How can we increase awareness about Covid 19 prevention in our city among the local population?

The general public must be made aware of the mode of transmission, presenting symptoms and the measures that can be undertaken to prevent the spread of infection. Few options- Media, Webinars...

03 January 2021 3,538 13 View

How to remove rotation along z-axis and shift the origin to body's center?

I have attached the files with this post to explain me how to remove rotation and center the origin at the body's local coordinate. This is just one sample and I have 1000s of files like this. I...

18 December 2020 3,838 3 View

How can we know the exact soil taxonomy of any particular site. Is there any online available database for Indian soil taxonomy based on geography?

Please If one can let know the soil taxonomy for vindhyan region falling in Mirzapur district of Uttar Pradesh, India.

14 December 2020 9,879 14 View

Does anyone knows a tool (GOMS based or other) to automate calculation of task completion time?

Hello good human, really hope that you are doing well. I have been reading "The Psychology of Human Computer Interaction" by Stuart Card et al. They have introduced one of the ground breaking...

11 December 2020 7,269 3 View

Is there a solvent for polypropylene at room temperature?

I want to dissolve polypropylene for making a solution so as to make fibres at room temperature. I have tried toluene and xylene but without any success in dissolving.

06 December 2020 8,479 2 View

How to upload the name of notable journal The Business & Management Review (Print) when it is not showing in dropdown list?

I am also unable to upload notable journal The Business & Management Review (Print) with ISSN 2047-2854 (Print) ISSN 2047-2862 (ONLINE) Indexing with: ProQuest, Ebsco Host, EconBiz,...

24 June 2020 3,125 3 View

A code in EES is required for simulating ejector cycle?

How value of P_m will be calculated by iteration? All values on LHS of equation 13 and 14 are known P_m, h_mix, u_mix and v_mix are unknown quantities See equation 13,14,15,16 w is also...

01 May 2020 5,313 1 View

Does anyone know a good IHC protocol for FGFR1 detection on floating brain sections?

I am trying to standardize IHC for cFOS and FGFR1 detection on rat brain sections. I use 40 micron sections and have had good results for cFOS detection. But I have not had any positive results...

25 March 2020 582 5 View

How to make a time series imputation benchmark ?

Hello ! In a work, I have to benchmark different algorithms to fill in missing values in time series. I insist on the fact that this is imputation and not forecasting. In my case, I have access...

24 February 2021 7,470 7 View

How to generate ECFP hashing folding data?

I'm trying to convert chemical structures to ECFP data. Buy, I have a problem about folding step. I understand all of the process about generating ECFP data though D. Rogers and M. Hahn's paper...

09 January 2021 7,454 2 View

Why train curves are being repeated in this five fold cross validation?

Hello, I am using ...... optimizer = RMSprop(lr=1e-4, decay=1e-6) N_FOLDS = 5 EPOCHS = 20 INIT_LR = 1e-4 Train_Batch_Size = 32 Valid_Batch_Size = 32 decay_rate = 0.95 decay_step =...

30 December 2020 2,519 3 View

How to change material in ANSYS & perform brittle deformation ?

I am currently working on a model for fracture generation due to pressure applied in ANSYS. When I am working with structural steel material it is showing only folding not any fracture initiation...

16 December 2020 3,826 3 View

Why accuracy over each k-fold corssValidation differ alot?

I have a data set with 91 Obervsation with 700 features. I have reduced the dimension of data using PCA? then I split the data set into 70/30. After that applied kfold =10 cross-validations on...

04 December 2020 1,259 4 View

How to get the most out of your qPCR data?

23 October 2020 7,537 3 View

Can I analyse NGS DATA from an EXCEL spreadsheet?

Hi, I have Fold expression of RNAseq data obtained from Ion Proton sequencer and got the fold change data with mean in an excel sheet. now can I analyse this fold change data using any...

20 October 2020 2,240 3 View

Which is the best way to implement 10-fold cross validation for CNN model?

I was performing a binary classification problem with 15000 RGB images using a scratch build CNN model. While it comes to evaluate the model, I can do it in two ways: 1. Splitting data Train...

08 September 2020 8,641 6 View

What is the best force field for proteins with folded and disordered regions?

Hello I want to do MD simulation on a protein model to refine its structure. My protein structure have both folded and intrinsically disordered regions. What force field should i choose for this...

23 August 2020 9,381 5 View

Shall we split the data into 60% of training and 40% of testing. And apply k fold cross validation for training and the best trained model for test?

Is the above method of splitting the data set into taring and testing, and then apply k fold cross validation for training, and then the trained model to classify the test set is right?

04 April 2020 9,040 3 View