What is a standard read out or assay to analyze the effect of dosages of Mitomycin C on cell lines? How is dosage standardization done for any cells?

I need to perform scratch assay to study migration of cancer cells upon various treatments of my molecule of interest. However, to block proliferation using mitomycin C, the dosage would vary for...

21 January 2018 4,565 1 View

How to plot fraction of the cell recovered v/s concentration of the drug ?

07 August 2017 619 1 View

How to Identify the interacting partner from whole cell protein ?

The gene X has been taken from host namely Z, cloned and expressed X in E.coli. I would like to understand the interaction partners of X protein in host A. As X is his-tagged protein, purified and...

04 May 2017 8,808 6 View

How to attain the desired dilution factor for particular CFU ?

Hello , I 'm having the total count of 1.75*10^13 CFU/mL of bacteria. For the assay, I need 5*10^5 to 1*10^6 CFU/mL. To understand the total dilution factor which I shall use for attaining the...

09 October 2016 6,182 6 View

Can anybody help me out with choosing the appropriate statistical test for analyzing my data ?

I have zebrafish embryo samples - control vs drug treated and I analyze the phenotype outcome of the drug treatment on certain neuronal populations (qualitative analysis). I have 15 fish embryos...

07 September 2016 8,121 8 View

Can I use Dasatinib for a short term TCR blocking functional assay?

I want to check for TCR mediated responses and when I use a TCR blocking antibody in my 9 hour assay it results in activation (my bad! ) so I was wondering if using dasatinib could be any good to...

24 February 2016 3,192 1 View

Why can I not see bands in my agarose gel after running my PCR product of sediment DNA?

crude DNA was1:10 diluted and used as template for PCR in a 50uL mixture and 4uL of the amplified product was mixed with 1uL of 6x gel loading buffer and run in a 0.8% agarose gel using 1x TE...

02 June 2015 1,304 10 View

How can one determine the ori site of plasmid is compatible with the bacteria of interest or not?

03 April 2015 6,355 5 View

Is it difficult to isolate membrane proteins from gram positive bacteira. and how the protocol will be very different from negative to positive

07 August 2014 6,813 1 View

What might be causing no amplification in PCR?

07 August 2014 6,877 21 View

Hello fellow researcher .. What is the meaning of your name?

I would like to create a small guide containing the meanings of the names .. Thanks to everyone who cooperates with me in achieving it.

03 March 2021 5,266 4 View

Is there a powerful system for the security of the systems distributed on IoT systems?

Is there a powerful system for the security of the systems distributed on IoT systems?

02 March 2021 3,858 10 View

How Relativistic effects is one reason for lanthanide contraction?

The Lanthanide contraction which is the decrease in ionic radii of the elements in the lanthanide series from atomic number 57, lanthanum, to 71, lutetium, is due to poor shielding of the 4f...

01 March 2021 2,272 4 View

Anyone that can help with details in phosphopeptides enrichment?

Anyone know the amount of protein before enzymatic digestion for phosphopeptides enrichment of serume sample in Ti-IMAC? Has anyone ever use Ti-IMAC(the material is from Chinese company named...

28 February 2021 7,310 3 View

Does the circuit modeling for planar monopole antennas using Foster canonical forms give good approximation?

Hi researchers, I am searching for some accurate way to do the circuit modeling of planar CPW-fed monopole antennas. I have gone through the literature where mostly circuit modeling is done using...

28 February 2021 7,016 1 View

For modelling epoxy-based matrices (in composites), which model in ABAQUS is the most suitable, Concrete damaged plasticity or Drucker-Prager model?

I am working on developing a micromechanical FE model for predicting kink-band formation in UD composites. To model matrix plasticity, which model, out of Concrete Damaged Plasticity and...

28 February 2021 5,415 1 View

How to get rid of somebody pretending to be the author of a publication?

One of the publication I co-authored has the author's name in abbreviation. Then, somebody whose nothing to do with that paper acquired it as her work. To whom in this RG shall I ask for a hand,...

27 February 2021 4,206 4 View

Is there a fast method matching citations to bibliography (manually typed references)?

I need to be able to match in-text citations to a reference list and back again in very large documents (100+ pages) WITHOUT the use of referencing software like Endnote. my method is to type the...

27 February 2021 9,848 1 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Can anyone share his experience regards Smoothing in origin?

I am working on TiO2 composite material and the results of XRD have a lot of small/junk peaks and while I apply a smooth filter in Origin then the other characteristics peaks disappear so anyone...

26 February 2021 8,183 3 View