Dhanashree Lokesh

4 Questions 13 Answers 0 Followers

Questions related from Dhanashree Lokesh

Can someone help or suggest alternative to LDH cytotoxicity assay in the particular condition mentioned below?

I have a molecule that changes the color of the media to yellow; when I was trying to use this for the LDH cytotoxicity assay, I had a lesser value than the test. As the compound was changing the...

17 January 2023 3,684 7 View

Purified membrane protein is getting precipitated while dialysis, Is there any solution to overcome?

I have purified a membrane protein via affinity purification using detergent OG. I was trying to dialyze the protein with ST (NaCl-150 mM and Tris-20 mM) buffer(pH 8). Unfortunately, during...

25 November 2022 1,847 12 View

Fold change calculation?

I have protein spectral counts data obtained after the MS/MS analysis with two conditions: temperature and treatment. What is the best way to calculate the fold change?

27 November 2021 8,197 2 View

How can I interpret the MIC data which I got through a plate assay?

Hello, I have done 96 well plate assay for detection of MIC of antibiotic for particular organism. I took the O.D of the plate and I understood the concentration of antibiotic which inhibits the...

31 December 2015 707 17 View