What is a standard read out or assay to analyze the effect of dosages of Mitomycin C on cell lines? How is dosage standardization done for any cells?

I need to perform scratch assay to study migration of cancer cells upon various treatments of my molecule of interest. However, to block proliferation using mitomycin C, the dosage would vary for...

21 January 2018 4,565 1 View

How to plot fraction of the cell recovered v/s concentration of the drug ?

07 August 2017 619 1 View

How to Identify the interacting partner from whole cell protein ?

The gene X has been taken from host namely Z, cloned and expressed X in E.coli. I would like to understand the interaction partners of X protein in host A. As X is his-tagged protein, purified and...

04 May 2017 8,808 6 View

How to attain the desired dilution factor for particular CFU ?

Hello , I 'm having the total count of 1.75*10^13 CFU/mL of bacteria. For the assay, I need 5*10^5 to 1*10^6 CFU/mL. To understand the total dilution factor which I shall use for attaining the...

09 October 2016 6,182 6 View

Can anybody help me out with choosing the appropriate statistical test for analyzing my data ?

I have zebrafish embryo samples - control vs drug treated and I analyze the phenotype outcome of the drug treatment on certain neuronal populations (qualitative analysis). I have 15 fish embryos...

07 September 2016 8,121 8 View

Can I use Dasatinib for a short term TCR blocking functional assay?

I want to check for TCR mediated responses and when I use a TCR blocking antibody in my 9 hour assay it results in activation (my bad! ) so I was wondering if using dasatinib could be any good to...

24 February 2016 3,192 1 View

Why can I not see bands in my agarose gel after running my PCR product of sediment DNA?

crude DNA was1:10 diluted and used as template for PCR in a 50uL mixture and 4uL of the amplified product was mixed with 1uL of 6x gel loading buffer and run in a 0.8% agarose gel using 1x TE...

02 June 2015 1,304 10 View

How can one determine the ori site of plasmid is compatible with the bacteria of interest or not?

03 April 2015 6,355 5 View

Is it difficult to isolate membrane proteins from gram positive bacteira. and how the protocol will be very different from negative to positive

07 August 2014 6,813 1 View

What might be causing no amplification in PCR?

07 August 2014 6,877 21 View

No title

02 March 2021 324 2 View

What is the value of Conference Review Paper?

How much value does a review paper carry in a higher study aspirant student's profile which is presented in a Peer Reviewed Scopus Indexed conference?

27 February 2021 2,068 3 View

Are you looking for a quality online conference on teaching and learning technology?

This large international conference will be held online this year due to COVID. I am submitting a paper to the Technology Enhanced Language Learning track (8). It is about a topic I have never...

15 February 2021 1,975 15 View

How to select from various invitations from Scientific Boards in conferences and proceedings?

How to select from various invitations from Scientific Boards in conferences and proceedings? How to spot the ones with high impact and genuine? Can we spot valuable events?

02 February 2021 8,187 1 View

What is the most appropriate design (statistic test) to assess the toxicity of 2 different compounds at 6 different concentrations and 2 conditions?

10 January 2021 8,077 3 View

Can Fecal Microbiota Transplantation from young heathy person to elderly persons improve health conditions?

06 January 2021 7,353 10 View

Are international conferences a dying species?

In our discipline (chemistry), annual meetings of the American Chemical Society (ACS) used to be mega-events with up to 20,000 participants. The current one will be virtual, and the organizers...

06 January 2021 1,882 5 View

Is it easy to collect a conference certificate?

share your experience please.

01 January 2021 5,084 4 View

Is training really prepare you for a conference?

Does training really prepare you for a conference?

01 January 2021 1,601 2 View

What is the procedure of attending international conference and seminar?

What is the procedure of attending international conference and seminar?

01 January 2021 7,733 1 View