What is a standard read out or assay to analyze the effect of dosages of Mitomycin C on cell lines? How is dosage standardization done for any cells?

I need to perform scratch assay to study migration of cancer cells upon various treatments of my molecule of interest. However, to block proliferation using mitomycin C, the dosage would vary for...

21 January 2018 4,565 1 View

How to plot fraction of the cell recovered v/s concentration of the drug ?

07 August 2017 619 1 View

How to Identify the interacting partner from whole cell protein ?

The gene X has been taken from host namely Z, cloned and expressed X in E.coli. I would like to understand the interaction partners of X protein in host A. As X is his-tagged protein, purified and...

04 May 2017 8,808 6 View

How to attain the desired dilution factor for particular CFU ?

Hello , I 'm having the total count of 1.75*10^13 CFU/mL of bacteria. For the assay, I need 5*10^5 to 1*10^6 CFU/mL. To understand the total dilution factor which I shall use for attaining the...

09 October 2016 6,182 6 View

Can anybody help me out with choosing the appropriate statistical test for analyzing my data ?

I have zebrafish embryo samples - control vs drug treated and I analyze the phenotype outcome of the drug treatment on certain neuronal populations (qualitative analysis). I have 15 fish embryos...

07 September 2016 8,121 8 View

Can I use Dasatinib for a short term TCR blocking functional assay?

I want to check for TCR mediated responses and when I use a TCR blocking antibody in my 9 hour assay it results in activation (my bad! ) so I was wondering if using dasatinib could be any good to...

24 February 2016 3,192 1 View

Why can I not see bands in my agarose gel after running my PCR product of sediment DNA?

crude DNA was1:10 diluted and used as template for PCR in a 50uL mixture and 4uL of the amplified product was mixed with 1uL of 6x gel loading buffer and run in a 0.8% agarose gel using 1x TE...

02 June 2015 1,304 10 View

How can one determine the ori site of plasmid is compatible with the bacteria of interest or not?

03 April 2015 6,355 5 View

Is it difficult to isolate membrane proteins from gram positive bacteira. and how the protocol will be very different from negative to positive

07 August 2014 6,813 1 View

What might be causing no amplification in PCR?

07 August 2014 6,877 21 View

Why should we choose deep learning to machine learning? Why is CNN better for image classification? Can I get Typical Answer?

What Characteristics makes CNN work better?

03 March 2021 1,458 4 View

What are some of the research gaps in machine learning and artificial intelligence in africa?

i would to know some of the research gaps in the artificial intelligence field in most african countries.

03 March 2021 6,145 3 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Anyone plz suggest relevant topic for research in image processing+Deep learning.?

I have selected brain tumor images ...but now found that already lots of research done n this topic.

03 March 2021 5,774 3 View

Irradiation data for JAPAN?

Hi, Could you help me to get the latest (2019, 2020) solar resource data for a particular site in JAPAN? PV GIS and NSRDB data are not in reach to JAPAN, I'm looking for some other database for...

03 March 2021 961 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Can you share me how teacher education is managed in your respective national educational system?

I feel that the practice in teacher education in my country is below the expected performance level due to very poor management system. Hope I will learn something from your experiences.

02 March 2021 1,516 4 View

Can NFL theorem be valid in infinite search space in ML?

NFL theorem is valid for algorithms training in fixed training set. However, the general characteristic of algorithms in expanded or open dataset has not been proved yet. Could you show your...

01 March 2021 1,189 3 View

What is the difference between L1 and L2 regularization in machine learning?

L1 and L2 regularization

28 February 2021 4,187 3 View

How to eliminate Steady state error from Reinforcement learning genetic algorithm code?

The following code (see 1st 2 images attached) is used to produce PID controller values that are designed to control the system (G). The code finds the PID controller values (noted as k) by using...

28 February 2021 6,560 14 View