Fluorescent dyes for efflux assay in fungi?

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How easy is it to use WinScanopy Camera system and software to take and analyze hemispherical photos?

Hi All, I am tempted to use WinScanopy software (by Regent Inc, Canada) to analyse some forest canopy pictures. However, I am new to this topic and have no experience with this. Form their website...

08 August 2022 5,388 0 View

Can anyone recommend a simple coding system for emotion expression in preschoolers?

I would like to examine young children's emotional expressions/ emotion-related behavior from video-recordings of an experimental problem-solving task. I'm looking for a simple way to code this...

03 October 2021 8,803 2 View

Which 96-well plates for LC-MS/MS?

Hi all, I'm looking for 96-well plates to be used for high-throughput on an LC-MS/MS system. Has anyone expierence which 96-well plates ? Which ones are are any good? Any recommendations for...

20 September 2020 6,140 4 View

HOMA-IR or TyG?

To establish insulin resistance, should HOMA-IR or the triglyceride glucose index (TyG) be used?

01 July 2020 5,664 1 View

Cutoff for too low reaction times?

Dear all Since quite a while now we are thinking about the following problem: we have a neuropsychological task in which participants see six figures and have to find the odd-one-out by pressing a...

04 March 2020 927 8 View

Have you had any experience with DM2 patients who suffer from diabetic cardiomyopathy and low carb diet?

I am about to write a research proposal for my PhD with Deakin University (Australia). The aim is to improve thirst, general well being and biomarkers ( related to heart failure ) in patients with...

22 May 2019 7,669 8 View

Is it possible to perform a repeated measure M-ANOVA?

Does someone know if I can perform for 1 uV with 4 ordinal categories and >2 interval scaled aVs a repeated measure (M)ANOVA? And if so, could you please recommend me literature/papers to learn...

30 August 2018 6,159 2 View

Which region of the gene should I target for a CRISPR mediated Knockout of PARP1?

I'm currently planning an experiment for knocking out PARP1 in a human cell line with either the Nuclease or Double Nickase system. I'm not sure which region of the gene would be the best to...

09 January 2018 7,240 4 View

Can anybody tell me how to attach murine islets of langerhans firmly on glass cover slides?

I´m looking for a good method to attach isolated murine islets firmly to glass cover slides (for FRET measurements or IHC). I´ve tried Laminin and Collagen, both do not really work... Thanks for...

10 April 2017 5,643 1 View

In a solvent system composed of water:isooctane:ethyl acetate:acetic acid (50:10:60:15) How the solvents react to each other?

Hi all!I'm using a system of solvent for TLC and lipids analysis (phosphatidic acid, phosphatidylcholine...). It is composed of water:isooctane:ethyl acetate:acetic acid (50:10:60:15).There is 2...

21 February 2017 939 2 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

Good Day, can I ask for your help on the questionnaire that I want to adopt?

I am currently looking for a questionnaire that relates with my study which is The Effectiveness of Organizational Culture towards employee retention rates

03 August 2024 3,505 6 View