Hi all!
I'm using a system of solvent for TLC and lipids analysis (phosphatidic acid, phosphatidylcholine...).
It is composed of water:isooctane:ethyl acetate:acetic acid (50:10:60:15).
There is 2 phases that are forming when I mix the solvents together. The upper phase is organic if I understand well. What I don't understand is what is the composition of the different phases and how the solvents react to each other?
Thank you for your help and your time!
You are right. The top part is the organic phase, mainly containing isooctane and ethylacetate. The bottom part is the aqueous phase, mainly containing acetic acid. However, both phase contains all four components more or less. It happens, but no reaction among them. If you want to get homogeneous solution, MeOH can be helpful.
Thank you very much for your help!
The Cyberlipid site is really good. This TLC solvent system is pretty common when you have to separate phospholipids after a phospholipase D activity assay. And I'm happy with my TLC results so far. I was just wondering if and how the different solvents are interacting. The fact that the upper phase contains the four components more or less is probably required for a good separation of the different compounds?
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