Hello Monica,

Fc blocking solutions are more efficient than BSA or FCS because they specifically target and block Fc receptor binding, whereas BSA and FCS provide general protein blocking, which can be less effective and may lead to non-specific binding. 

Fc Blocking reagents are superior in terms of specificity as they target Fc receptors directly, ensuring that only antigen-specific binding is observed, minimizing on non-specific antibody binding, resulting in cleaner and more accurate results.

So, in a way, you will have improved sensitivity with less background, becoming easier to detect and analyze specific cell populations or antigen.

Please note that for flow cytometry experiments, prevention of non-specific binding is essential to ensure accurate cell labeling and analysis. 1% BSA or 1% FCS in PBS can also perform the task but not that efficiently.

Best,

Hello Mónica, how are you? I would use a Fc blocking solutions.

Hi Monica. I agree with people above. Some articles even mention that Fc blocker doesn't block FcR enough and you need more blocker, to which I have same impression. I recommend to use serum solution. If the species of your antibodies is mouse / rat, mouse/ rat serum can be used as a blocker.

I usually make blocking buffer containing 10% mouse serum in 1% BSA.

Hope this helps.