Ken Yasaka

2 Questions 3 Answers 0 Followers

Questions related from Ken Yasaka

Why can't my antibodies detect the protein in the transfected cells, while DDK-tag is detected?

I am trying a transfection assay using HEK293 cells and wanna validate the transfection in flow cytometry. The plasmid contains the sequence of the protein and myc-DDK tag. Transfection is a...

01 April 2025 6,234 3 View

Does the stimulation of PBMCs see the decrease in B cell fraction?

Hi, I am doing the culture PBMCs from healthy humans in which B cells are stimulated by anti-Ig and TLR7 ligand. Very oddly, on day2, I see dramatic decrease in the ratio of B cells compared to...

08 January 2025 9,153 0 View