Protein lysates are more stable when you quick freeze them in liquid nitrogen. To my knowledge during slow freezing a density gradient is formed that can lead to protein precipitation and denaturation, but is it better for the CCC form of the plasmid to freeze it quickly or slowly? I heard that because of the torsion forces of the structure it is better to freeze it slowly - is this a true statement?
Philipp Bartsch
Quick freezing using liquid nitrogen comes to smaller ice crystals compared to slow freezing. Hence I would guess that quick freezing causes less stress to your supercoiled plasmids. Nevertheless I normally put my plasmids in the freezer (–21 °C) and let them freeze "slowly" and I haven't had any problems up to now.
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