How to handle intensive longitudinal data with R, when having three intervention groups and 98 daily measurement points for each individuals (21) ?

I collect data of 21 participants daily over 14 weeks (98 measurement points per participant/physiological data). Each participant is part of an intervention group. All interventions have the...

28 July 2019 3,880 7 View

How to make sure that the No. of differentiated SH SY5Y cells in equal in XTT assay?

I would like to perform an XTT assay with differentiated SH SY5Y cells in 96 wells. However, although I will plate the exact number of cells in each well, I cannot assume that by the end of the...

28 November 2018 9,516 4 View

Any ideas for a protocol or extraction kit for extracting DNA from tomato fruit for the study of bacterial microbiota using NGS?

I'm trying to extract DNA from tomato fruit in order to study the bacterial microbiota found inside the fruit using NGS. I have tried using a CTAB protocol, but the precipitate is highly charged...

20 April 2018 423 3 View

Different results between MANOVA and moderated Mediation with PROCESS?

have to calculate a moderated mediation with 2 mediators and 2 dependent variables. My model: predictor (dichotomous)moderator (dichotomous)both mediators (continous)both dependent variables...

18 September 2016 5,657 2 View

Moderated Mediation: different results between stand. & unstand. coeff.?

Hi, I'm testing a moderated mediation with two independent variables which are dichtomous (one of them is my moderator), two mediators (interval scale) and one dependent variable. I'm using...

12 September 2016 9,639 3 View

What aspect of crystallinity influences cell attachment?

Crystallinity is a term loosely used to represent a disordered material. For thermally sprayed coatings, this is usually referred to the amorphous phase relative to the total (amorphous +...

02 December 2014 1,018 3 View

Does anyone have a protocol on how to perform a tharacic spinal cord transection in rats?

I am looking for a method to perform a spinal cord transection in an in vivo anesthetized rat model. This will be nonsurvival surgery, so after the spinal cord transection the rat will be...

27 February 2013 6,650 3 View

How to get HeLa cells from adherent culture into suspension culture?

Hi, I would like to grow HeLa cells in suspension since you can grow up more culture then as adherent cells. Therefore, I am wondering what I need to do with my HeLa cells which are growing...

16 April 2012 1,815 13 View

How to get HeLa cells from adherent culture into suspension culture?

Hi, I would like to grow HeLa cells in suspension since you can grow up more culture then as adherent cells. Therefore, I am wondering what I need to do with my HeLa cells which are growing...

03 April 2012 7,229 14 View

Does a knockdown of p53 in luminal epithelial cells support their growth or might even drive them into tumorigenesis?

Hello everyone, I would like to know if anyone knows if a knockdown of p53 in primary luminal epithelial cells of mouse mammary glands does support the growth of these cells in cleared fat pad...

03 April 2012 6,661 83 View

What is the characteristic length to determine the Reynolds number for the scattered turbulent fluid flow?

Dear all, I just saw this example in FB on the difference between laminar flow and turbulent flow. The flow from a pipe hits the sink and scattered. The flow from the pipe is characterized as...

02 March 2021 1,999 5 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Why do the air flow results vary totally when adding scalar in CFD ?

Dear All, We are using Autodesk cfd to calculate the air flow in a single sided naturally ventilated room, where air flow depends only on buoyancy due to temperature difference between inside and...

25 February 2021 9,602 4 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

Why anti-CD20 or other antibodies are not used to label target cells in flow cytometry t-cell cytotoxicity assay?

New to the field of cell therapy. Why fluorescent-labeled antibodies to target cell antigens are not used for determining target cell count by flow cytometry in cytotoxicity assay (eg car-t)? Cell...

21 February 2021 3,278 3 View

How to calculate time-averaged fields from Ansys CFX solver?

I would appreciate any comments on the following problem. I need to calculate mean fields (e.g. pressure, velocity and vorticity), for which I activate Trn Stats tab in CFX-Pre. However, what I...

21 February 2021 8,548 7 View

Which software is best to analyse multi-phase fluid flows ?

Hello everyone, hope all is good. I want to analyse multi-phase fluid flow through pipe, which software is best to do so ?

21 February 2021 4,230 8 View

Can anyone help with an Accurate Diffuser Expansion Loss formula for rectangular shaped diffusers in turbulent flow regime?

I have tried Idelchik's Handbook of Hydraulic Resistance 4th Edition (2007 page 300) formulas and across some range the formulas are reported valid for, the law of conservation of energy is...

20 February 2021 2,504 1 View

Oil red O protocol for flow cytometry?

18 February 2021 4,214 3 View

How to read a flow cytometry graph detailing cell cycle?

Hello everyone, When determining cells in different stages of the cell cycle, cells are stain for Brdu and Propidium Iodide. I understand how PI can be used to discriminate between G1 and S/G2...

18 February 2021 3,920 3 View