Hello everyone, I would like to ask if anyone know a surface antigen on mouse fibroblast cells which can be used for FACS to separate these from epithelial kidney cells? Any help would be grateful! Thanks in advance, Nicole
Hi All, I have a small question regarding the random-intercept cross-lagged model (RI-CLPM). Is it possible to find out, whether a positive significant cross-lagged effect implies that a positive...
22 May 2022 6,932 0 View
As the question states, I am currently doing some revisions for a paper on personal branding for artists. Although I have found enough theory in the influencer marketing area, I am interested in...
17 April 2022 2,125 7 View
I address this dilemma to historians and anthropologists. Is it or is it not beneficial to colorize archival photographs using artificial intelligence? When I first discovered this possibility it...
06 April 2022 9,575 4 View
Can I prepare cell media with 2-deoxyglucose and sue it several days in a row or do I have to add 2-DG from a frozen stock every time?
20 June 2021 8,899 1 View
I have questions regarding measurement invariance for longitudinal data when analyzing latent variables. I would like to analyze 4 cohorts (grades 3,4,5,6 at baseline) longitudinally over 2 years...
11 May 2021 1,668 15 View
I am developing a paper where I want to address issues concerning social environmental indicators and governance in companies.
30 April 2021 9,009 3 View
I'm wondering if there are "classical" bacterial outer membrane vesicles markers similar to tetraspanins (CD9/CD63/CD81) used for human/animal cell exosomes that could be detected by WB or ELISA...
28 March 2021 3,262 4 View
I'm culturing lymphocytes (i.e. cells in suspension, 6-14um in diameter) for a period of 2-3 weeks in the same 6-well plates (volume of 2-4mL per well). Throughout the culture period I add target...
14 March 2021 9,572 3 View
I want to establish a 3D culture of mouse peritoneum and want to use a valid mesothelial cell line (not a primary one). I found just human mesothelial cell lines (like LP9). Do you familiar with a...
10 April 2020 5,115 0 View
I am performing a B-gal assay and need to counterstain. Does anyone have experience doing this with neutral red and what concentration/solvent do you make the NR up in? Thanks!
22 October 2019 3,323 0 View
To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...
06 August 2024 6,728 6 View
Women, on the other hand, can become physically aroused (increased blood flow in the reproductive organs) without becoming psychologically aroused even in the slightest. (Robert Weiss)
05 August 2024 9,537 2 View
We have a lateral flow test on Sartorius 140 NC. The conjugate is gold-monoclonal antibody. Using different control lines (GAM, protein A, protein G) we get a very strong leading edge with reduced...
31 July 2024 4,510 3 View
Hello everyone, I am currently using washed human platelets to stain Annexin V as a procoagulant marker. Additionally, I am staining with PerCP-CD61 to identify platelet cells. So far, I have...
29 July 2024 8,624 1 View
I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...
28 July 2024 3,178 3 View
Hello; I am conducting a CFD study on two-phase flow in a separator, utilizing refrigerant as the working fluid. For this study, I have implemented pressure inlet and pressure outlet boundary...
25 July 2024 2,309 5 View
Hi everyone, I am working on a curved domain in which a ship is situated in the middle (geometry is given below). In my understanding the general fluid flow is parallel to the x axis from inlet to...
25 July 2024 9,058 4 View
Hello, I'm conducting flow cytometry experiments using the BD Cell Viability Kit and I've noticed that the TO solution ends much faster than the PI solution. I store the solutions according to...
22 July 2024 2,257 0 View
I am giving starvation stress to the soil amoeba (Dictyostelium discoideum) cells in my study groups and using NAC as a negative control. I am using DHE for ROS measurement.
20 July 2024 6,354 0 View
In terms of CFD, we often analyze the stability of the error using Von-Neumann analysis, especially for FDM based problems. Should we follow the same approach for a compressible fluid flow using FVM ?
13 July 2024 6,296 5 View