I am performing a B-gal assay and need to counterstain. Does anyone have experience doing this with neutral red and what concentration/solvent do you make the NR up in?
Thanks!
I want to sequence the genome of Chilean powdery mildew isolates from grape leaves using the Oxford Nanopore platform. Thanks in advance for your kind response.
17 April 2024 4,863 2 View
Doing research on how the treaty of Versailles led to secularism in Europe
24 February 2024 4,696 3 View
I am using document analysis to explore how Christian philosophies of education are presented.
14 February 2024 9,867 2 View
We have 500+ pt data collected and would like to seek a freelance biostatistician who can assist with the analysis.
11 April 2023 5,090 4 View
I am running into issues with trying to score the PBR Avoidance Social, PBR Avoidance Physical, PBR Humor/Distraction, PBR Pain Persistence, and PBR Behavioral Endurance sub-scales. There is a...
04 June 2022 8,465 1 View
Hi All, I have a small question regarding the random-intercept cross-lagged model (RI-CLPM). Is it possible to find out, whether a positive significant cross-lagged effect implies that a positive...
22 May 2022 6,932 0 View
As the question states, I am currently doing some revisions for a paper on personal branding for artists. Although I have found enough theory in the influencer marketing area, I am interested in...
17 April 2022 2,125 7 View
I address this dilemma to historians and anthropologists. Is it or is it not beneficial to colorize archival photographs using artificial intelligence? When I first discovered this possibility it...
06 April 2022 9,575 4 View
Can I prepare cell media with 2-deoxyglucose and sue it several days in a row or do I have to add 2-DG from a frozen stock every time?
20 June 2021 8,899 1 View
I try to determine the Tafel constants as well as the exchange current density from potentiodynamic current density-potential curves for an electrolyte mainly composed of 0.3M CuSO4 and 1.9M...
12 May 2021 3,998 2 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
I need to do some experiment with it in liquid form.
07 August 2024 613 3 View
Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...
06 August 2024 5,801 4 View
I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...
03 August 2024 4,725 2 View
Hello everyone, I need to dissolve stigmasterol for an animal experiment, but it hasn't dissolved in either DMSO or vegetable oil. Can anyone recommend the best solvent for stigmasterol?
01 August 2024 7,504 2 View
Hello, colleagues! There is commenting open for new upcoming edition of USP 1033. Validation target acceptance criteria is now different from what it used to be and it doesn't include Cpm....
23 July 2024 7,292 3 View
Why methanol and sulphuric acid, used in the analysis of polyhydroxyalkanoates (PHA) methyl esters by GC-MS? Additionally, why do we typically use non-polar solvents in GC-MS?
22 July 2024 1,210 2 View
Is it the best optimization ratio based on experiments?
22 July 2024 8,170 0 View
If you don't understand my question let me know
21 July 2024 563 1 View
when we grow biofilm in test tube, the pipette often do not reach near the content. so we have to decane it directly or use a smaller test tube.
17 July 2024 3,532 0 View