Is 2-deoxyglucose stable in media?

21 June 2021 1 9K Report

Can I prepare cell media with 2-deoxyglucose and sue it several days in a row or do I have to add 2-DG from a frozen stock every time?

Sabri Hasan

excuse me its not under my specialize

Badges
Science topic

More Golda Gross's questions See All

Jakie były losy polskiego tłumacza Marcelego Tarnowskiego? / Do you have any information about the life of Polish translator Marceli Tarnowski?

Mgr Paweł Golda (Uniwersytet Śląski w Katowicach, Uniwersytet Opolski, Université Sorbonne Paris Nord) i mgr Joanna Ryszka (Uniwersytet Śląski w Katowicach, Uniwersytet Rzymski „La Sapienza”),...

27 July 2023 7,257 0 View

Implications of a positive significant cross-lagged effect in a random-intercept cross-lagged model (RI-CLPM)?

Hi All, I have a small question regarding the random-intercept cross-lagged model (RI-CLPM). Is it possible to find out, whether a positive significant cross-lagged effect implies that a positive...

22 May 2022 6,932 0 View

Personal Branding for artists. How can artists benefit from social media platforms?

As the question states, I am currently doing some revisions for a paper on personal branding for artists. Although I have found enough theory in the influencer marketing area, I am interested in...

17 April 2022 2,125 7 View

Considerations on automatic photographic recolorization of archival photographs. Is it beneficial for historians and anthropologists?

I address this dilemma to historians and anthropologists. Is it or is it not beneficial to colorize archival photographs using artificial intelligence? When I first discovered this possibility it...

06 April 2022 9,575 4 View

Is it necessary to test for configural, metric, scalar as well as strict invariance 1) longitudinally 2) between cohorts for each measurement point ?

I have questions regarding measurement invariance for longitudinal data when analyzing latent variables. I would like to analyze 4 cohorts (grades 3,4,5,6 at baseline) longitudinally over 2 years...

11 May 2021 1,668 15 View

Can anyone recommend paper on environmental social and governance (ESG) performance indicators?

I am developing a paper where I want to address issues concerning social environmental indicators and governance in companies.

30 April 2021 9,009 3 View

How to detect bacterial OMVs?

I'm wondering if there are "classical" bacterial outer membrane vesicles markers similar to tetraspanins (CD9/CD63/CD81) used for human/animal cell exosomes that could be detected by WB or ELISA...

28 March 2021 3,262 4 View

How to filter out debris from culture wells?

I'm culturing lymphocytes (i.e. cells in suspension, 6-14um in diameter) for a period of 2-3 weeks in the same 6-well plates (volume of 2-4mL per well). Throughout the culture period I add target...

14 March 2021 9,572 3 View

Which mouse mesothelial cell line should I use for peritoneum 3D culture?

I want to establish a 3D culture of mouse peritoneum and want to use a valid mesothelial cell line (not a primary one). I found just human mesothelial cell lines (like LP9). Do you familiar with a...

10 April 2020 5,115 0 View

Counterstaining with Neutral Red?

I am performing a B-gal assay and need to counterstain. Does anyone have experience doing this with neutral red and what concentration/solvent do you make the NR up in? Thanks!

22 October 2019 3,323 0 View

Shortage Cost Hypothesis?

What can be a hypothesis related to the Stock out Cost or Shortage Cost?

29 July 2024 9,259 2 View

Standard curve of H2O2?

Hi, I need to prepare a standard curve of H2O2 to evaluate the primary products of oxidation. H2O2 I have is 35%. How do I prepare a stock of H2O2 10 microlitere. If s.o has experience, I would be...

26 July 2024 3,460 2 View

How to prevent microbubbles during immunofluorescence imaging?

Hi ResearchGate community, how are you? I need to request your help to mitigate the generation of microbubbles detected during the slide preparation of sample metaphase chromosomal spreads. I...

01 July 2024 7,694 4 View

What are impact of stagflation on emerging markets performance?

The emerging markets have suffered from a state of stagflation. I hope you know your expectations according to your experiences regarding the implications for the stock markets,,,, risks and...

19 June 2024 4,410 3 View

Why is iPSC in bad condition?

I saw the iPSC about a week after holding the stock (12 well and 3 stocks) and there were very few cells attached, and some cells were differentiated. I wonder what the problem is. The plate is...

12 June 2024 6,087 0 View

I am working on antimicrobial effect of CBD (Cannabidiol), how should I prepare the stock which dissolve in Muller Hinton agar?

I am working on antimicrobial effect of CBD (Cannabidiol). As it is insoluble in water, I prepared a stock of 500 mg/ml (in DMSO). Now I have two problems: 1- I can not dissolve more than 500 mg...

10 June 2024 9,564 6 View

Does anyone know where one can buy 8161 glass capillaries for making patch pipettes?

Warner used to sell 8161 glass, but Harvard Biosciences bought Warner and doesn’t appear to stock it anymore

04 June 2024 5,688 2 View

What is the best way to store phage stock (phi11) for long durations?

My lab doesn't use phage often and recently ran into a problem with too-low of titer for our phi 11 (to the point where I couldn't get it to lyse after 3 and 24 hours). We make a lysate prep,...

27 May 2024 7,900 1 View

How to dilute my DNA Template( 1 Micromolar stock solution ) into1 × 10-8 nM to 1 × 10-15 nM ?

How can i dilute my master stock of 1 micromolar DNA template to different concentration by serial dilution

17 May 2024 3,641 2 View

How to dilute stock solution to add to cells in 96 well and 6 well plates?

Most times after checking the cyctotoxity, my results are not consistent. But i used the same concentration of my stock solution. Can anyone help out with how to determine the concentration of my...

09 May 2024 1,534 2 View