How to handle intensive longitudinal data with R, when having three intervention groups and 98 daily measurement points for each individuals (21) ?

I collect data of 21 participants daily over 14 weeks (98 measurement points per participant/physiological data). Each participant is part of an intervention group. All interventions have the...

28 July 2019 3,880 7 View

How to make sure that the No. of differentiated SH SY5Y cells in equal in XTT assay?

I would like to perform an XTT assay with differentiated SH SY5Y cells in 96 wells. However, although I will plate the exact number of cells in each well, I cannot assume that by the end of the...

28 November 2018 9,516 4 View

Any ideas for a protocol or extraction kit for extracting DNA from tomato fruit for the study of bacterial microbiota using NGS?

I'm trying to extract DNA from tomato fruit in order to study the bacterial microbiota found inside the fruit using NGS. I have tried using a CTAB protocol, but the precipitate is highly charged...

20 April 2018 423 3 View

Different results between MANOVA and moderated Mediation with PROCESS?

have to calculate a moderated mediation with 2 mediators and 2 dependent variables. My model: predictor (dichotomous)moderator (dichotomous)both mediators (continous)both dependent variables...

18 September 2016 5,657 2 View

Moderated Mediation: different results between stand. & unstand. coeff.?

Hi, I'm testing a moderated mediation with two independent variables which are dichtomous (one of them is my moderator), two mediators (interval scale) and one dependent variable. I'm using...

12 September 2016 9,639 3 View

What aspect of crystallinity influences cell attachment?

Crystallinity is a term loosely used to represent a disordered material. For thermally sprayed coatings, this is usually referred to the amorphous phase relative to the total (amorphous +...

02 December 2014 1,018 3 View

Does anyone have a protocol on how to perform a tharacic spinal cord transection in rats?

I am looking for a method to perform a spinal cord transection in an in vivo anesthetized rat model. This will be nonsurvival surgery, so after the spinal cord transection the rat will be...

27 February 2013 6,650 3 View

How to get HeLa cells from adherent culture into suspension culture?

Hi, I would like to grow HeLa cells in suspension since you can grow up more culture then as adherent cells. Therefore, I am wondering what I need to do with my HeLa cells which are growing...

16 April 2012 1,815 13 View

How to get HeLa cells from adherent culture into suspension culture?

Hi, I would like to grow HeLa cells in suspension since you can grow up more culture then as adherent cells. Therefore, I am wondering what I need to do with my HeLa cells which are growing...

03 April 2012 7,229 14 View

Does a knockdown of p53 in luminal epithelial cells support their growth or might even drive them into tumorigenesis?

Hello everyone, I would like to know if anyone knows if a knockdown of p53 in primary luminal epithelial cells of mouse mammary glands does support the growth of these cells in cleared fat pad...

03 April 2012 6,661 83 View

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

Diltuions for a Western blot?

I need help using the equation C1V1=C2V2 figuring out how much antibody to add to use for a western blot. The stock was origianlly 3.45 ng/ul but diluted 1:10 so now its 0.345 ng/ul. I need to run...

22 February 2021 9,901 3 View

What are alternatives for B27+ Supplement in neuronal cell culture?

Hello everybody In our iPSC-derived neuronal culture we use Maturationmedium, supplemented with B27+. As Thermo Fisher run out of stock and can deliver earliest in May, I wanted to ask whether...

16 February 2021 3,348 1 View

How to revive a Hyphomicrobium strain?

11 February 2021 3,524 2 View

Bradford assay for insulin: discrepancies between standard curve and sample concentration during crystallisation?

Hi there, I would like to determine the unknown concentration of human insulin in my sample during crystallisation (the concentration range is roughly 0.5 -2 mg/ml, whereas the initial...

06 February 2021 9,370 7 View

How to measure volume of Pinus roxburghii ?

I want to measure volume of Pinus roxburghii in the Jammu region of India to calculate the carbon stocks of Pinus roxburghii.

31 January 2021 2,311 3 View

Where can I find the information about steel final production in Japan?

I am doing research about the in-use stock of iron and steel in Japan. I need the report about annual yield of steel final production. I found some information in the webpage of Japan Iron and...

25 January 2021 3,867 2 View

How to revive bacteria from glycerol stock?

19 January 2021 7,025 4 View

How can I prepare the erythromycin stock?

I need to prepare a stock of 10 mg / ml erythromycin to check the mic value. I am trying to dissolve 0.1g/10 ml of water but i can't dissolve erythromycin in water. How can i prepare 10 mg/ml...

03 January 2021 9,947 3 View

Which form of tryptophan and tyrosine is highly soluble in water?

The solubility of tryptophan and tyrosine in water is low. Is there a very highly soluble structurally similar or derivatives of these amino acids I can use? it doesn't matter if it is...

20 December 2020 3,157 3 View