Thanks Jimmy. I tried TGF beta1 treatment, but couldn't up regulate mesenchymal marker like vimentin and Ecadherin down regulation was very minimum, checked by flowcytometry.
You could try adding in nutrient deprivation and or hypoxia? And Vimentin is a very good marker of emt so I would try a different marker. Try a transcription factor like snail or zeb
Thanks Jimmy, I was doing serum starvation along with TGF beta treatment, and in addition to vimentin also checked fibronectin but couldn't see any up regulation. Any way will try snail or zeb. thanks a lot.