Does anyone know EpCam negative breast cancer cell line?

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EMT

Can anybody suggest a way to induce in vitro EMT in breast cancer cell lines?

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Can we classify HV and LV lines based on line length?

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No title

02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

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Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

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Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

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Which cell line should we use to perform biological dosimetry experiments?

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Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

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Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

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Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

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What could be some reasons why unstimulated monocytes are getting activated?

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Agnieszka Martowicz

Hs578t. And MDA-MB-231 has low EpCAM expression.

Amirali Bukhari

Even Zr-75-1 has low expression of EpCAM. However, I have not come across an EpCAM negative breast cancer cell line as of yet.

Sharif U Ahmed

I agree with you Bukhari. Thanks all.

Anne Grosse-Wilde

Interesting that there is nearly no breast cell line without EPCAM... why is that?

Weinberg's HMLER cells are EPCAM negative and Weinberg is great in sending them out. HMLER cells are derived from human mammary epithelial cells and immortalized and transformed iwth SV40LT and oncogenic RAS. And these cells have NOT undergone the huge selection pressure that metastatic cancer cell lines have gone through. I think this is a great cell line as HMLER cells are very heterogeneous and have stable and sortable populations of cells with different phenotypes, AND they still contain the population of CD24+/CD44- cells which is highly plastic but that is irreversibly lost in most other basal cell lines.