JUN and ABY dye are not available in Biorad machine. Can any one tell me which dye (present in biorad machine) wavelength is similar with JUN n ABY ??

For covid19 diagnosis TaqPath kit is having ABY n JUN dye... But in our lab Biorad Cx96 machine is available where ABY n JUN dye are not available. How can we use the Biorad machine for covid19...

22 July 2020 1,978 2 View

Is it possible to find the sensitivity of RMSD and RMSF in MD simulation through gromacs ?

Is it possible to find the regions of the protein structures most susceptible to temperature impact in terms of RMSD and RMSF , during MD simulation ?

06 May 2018 8,855 3 View

Can anyone suggest me where and how to apply for post-doc as my work is on utilization of Water Hyacinth

26 July 2017 3,040 3 View

What should be the ideal settings for HSP length cut-off and HSP-Hit coverage for running Blast in Blast2Go?

I have to analyze PacBio transcriptome data with four bin sizes: 1-2 Kb, 2-3 Kb, 3-6 Kb and 5-10 Kb. I can understand that the HSP length cut-off and HSP-Hit coverage settings must be customized...

26 July 2017 1,346 1 View

Can anyone identify the two plant species

The plants were collected from Barak Valley Assam

24 May 2017 9,971 6 View

By which means can the porosity be determined in a vegetal fibre ?

We develop a new mechanical characterization test for vegetal fibres. We need to know the apparent density of them. We can know easily the real density by pycnometry. We want to determine the...

12 April 2016 7,592 8 View

What are the advantages and the drawbacks of AFM and nanoindentation ?

I need to measure the mechanical properties of a composite material on its surface (so there are several material phases). Which method is better between AFM and nanoindentation ?

03 September 2013 2,030 9 View

Sample making for ELISA

At first I treated interferon beta protein to the tumor bearing mouse then I collected the tumor and blood from the mouse. I already made the tumor sample with PBS and collected serum from blood....

01 January 1970 7,883 4 View

ELISA sample preparation

I want to check interferon beta protein concentration in mouse tissue sample like heart, liver, lung and kidney. Can anyone please help how can I make the sample from the tissue for ELISA?

01 January 1970 1,848 3 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

Can I use a protein-devoid condition as my negative control in ELISA?

Hello, I am running a sandwich-type ELISA where I am trying to inhibit a protein-protein interaction using small molecules. For each experiment, I determine my Z' (Z-prime) to determine the...

23 February 2021 7,429 1 View

Surprisingly low antibody titers against SASRS-COV-2 RBD in hamsters compared to mice?

21 February 2021 6,958 2 View

Can anyone advise me about a cortisol antibody made in rabbit to set up an ELISA?

I am trying to set up a cortisol EIA using mouse anti-rabbit IGg as coating and a rabbit cortisol antibody as secondary ab. I am searching for a sensitive and specific cortisol Ab made in rabbit

20 February 2021 3,366 5 View