I am trying to isolate microbial DNA from soil samples stored at -80ºC ( one year old stored sample) using Nucleospin soil kit (Make: Macherey Nagel) but unable to get the DNA from stored samples even after several attempts. However, freshly collected samples are yielding good quantity of DNA from same kit. kindly suggest me, how can I get microbial DNA from stored samples, and how long we can store soil samples for metagenomics.
@ Lata, distribution of microbial taxonomy remains constant and allows for a reliable quantitative analysis for at least 6 months upon sampling when samples are kept at –80°C (Carroll et al., 2012). Moreover, high-quality DNA suitable for a 16S analysis was obtained from samples stored at –80°C for 14 years (Kia et al., 2016).
From my experience, the 1 year old sample is not a problem.
You might need to adjust your paramters or you can try Dneasy Powersoil pro Kit. I would recommend you to try an experiemnt with fresh soil sample, In which you keep your soil in -80 for 2-3 days, after that you need to try bacterial culture from the soil and at the same time you can go forward with the DNA isolation from soil, changing incubation and lysis time. Perform some experiments then you will find your right conditions.
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