ELISA, Immunoprecipitation and Affinity Chromatography

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ELISA and Hybridoma

Antibody – antigen interactions might involve all types of chemical bonding, except which one? Explain.

07 August 2017 2,293 5 View

Western Blotting

Why must 1. electrophoresis of the protein sample, and 2. transfer to nitrocellulose precede the actual probing of the sample with an antibody?

07 August 2017 9,508 1 View

All immunogens are antigens, but not all antigens are imunogens.?

Explain the statement “All immunogens are antigens, but not all antigens are imunogens.”

07 August 2017 4,712 7 View

ELISA and Immunohistochemistry

Why Immunohistochemistry requires a conjugated enzyme, whereas immunofluorescence does not. Explain the difference?

07 August 2017 945 5 View

ELISA and Hybridoma Technology

An investigator wanted to produce rabbit antiserum specific for mouse IgG, so she injected a rabbit with purified mouse IgG. However, to her dismay, the antiserum reacted with all isotypes of...

07 August 2017 8,415 7 View

ELISA

I am planning to run an ELISA soon but I have a question regarding sample preparation. If I don’t know the working range of my samples for the ELISA, should I just add the samples to the plate...

07 August 2017 5,462 4 View

Western Blotting and ELISA

What is the function of the enzyme bound to the secondary antibody?

07 August 2017 8,198 9 View

Dilution of Samples in ELISA

What is the need for serial dilution of the sample when performing an Elisa?

07 August 2017 1,692 1 View

Western Blot, ELISA and Immunohistochemistry

What are the differences and similarities between western blotting and immunohistology?

07 August 2017 2,178 3 View

What are the different types of ELISA?

The indirect method requires use of a ‘secondary’ antibody, whereas the direct methodology does not. Explain the difference.

06 July 2017 3,047 4 View

Which type of compound does lamda max of 218 indicate in a uv-vis spectrum of a partially purified compound through column and TLC?

A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...

11 August 2024 9,801 2 View

Can you connect an HPLC to a Mass Spec only at a certain time point?

Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...

11 August 2024 8,141 3 View

GC-MS retention index prediticon?

Hello experts, Does anyone know any free software about retention index prediction ?

08 August 2024 7,403 2 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

Do I need specific antibodies to stain for a fusion protein?

I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...

07 August 2024 5,338 2 View

How to determine positive-stained cells in FACS? Use isotype or unstained control?

To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...

06 August 2024 6,728 6 View

Are there any commercially available Donkey anti-Alpaca secondary antibodies?

Are there any fluorescently labeled anti-Alpaca secondary antibodies raised in Donkey? So far I have only been able to find anti-Alpaca secondaries raised in Goat. Or is this not possible due to...

04 August 2024 4,255 1 View

Why is TLC not a form of partition chromatography?

Why is TLC not a form of partition chromatography and paper chromatography not a form of absorption chromatography?

03 August 2024 7,035 3 View

Is there any Indian Company or Startups, from any universiy or IITS, which manufacture Gas Chromatography instrument , i want to make a custOmized G?

or we can collaborate

01 August 2024 6,705 2 View

Is there a good freeware software for photodocumentation of TLC?

I need something like Camag's VisionCat software for photodocumentation of TLCs. Could you recommend something?

31 July 2024 8,415 1 View

Gertrudis Rojas

Low pH, 2.5 or 3, is commonly used for elution in affinity chromatography. Of course, the method of choice depend on what kind of experiment are you trying to do.

Saleh Alkarim

By changing the pH or the salinity . The guide and link here will help you

https://courses.lumenlearning.com/microbiology/chapter/detecting-antigen-antibody-complexes/

Flavio Dolores Martínez-Mancera

With abrupt changes in the pH (>3) or salinity of the medium, following of a extenous purification procedure; more info try to visit invitrogen text books, regards!

Shrikant Patil

Thank You So Much!!!